Phenotypic characterization, osteoblastic differentiation, and bone regeneration capacity of human embryonic stem cell-derived mesenchymal stem cells.
Phenotypic characterization, osteoblastic differentiation, and bone regeneration capacity of human embryonic stem cell-derived mesenchymal stem cells.
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DOI：
10.1089/scd.2008.0310
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发表时间：
2009-09
影响因子：
4
通讯作者：
Krebsbach PH
中科院分区：
文献类型：
--
作者：
Arpornmaeklong P;Brown SE;Wang Z;Krebsbach PH
为了加深对人胚胎干细胞分化模式和成骨能力的了解，我们测定了人胚胎干细胞来源的间充质干细胞(hESCs-MSCs)向成骨细胞分化的时间模式，三维基质对长期培养的hESCs-MSCs成骨分化的影响，以及hESCs-MSCs来源的成骨细胞在颅骨缺损中的成骨能力。HESCs-MSCs在成骨介质中培养，诱导hESCs-MSCs向成熟成骨细胞分化，分化时间与人骨髓基质细胞和原代成骨细胞相似。通过在三维胶原支架上培养细胞来促进成骨分化。对表达碱性磷酸酶的细胞进行荧光活化细胞分选，获得丰富的成骨细胞群，用于体内移植。绿色荧光蛋白的鉴定和人特异性核抗原在新生骨细胞中的表达证实了移植的人细胞在骨再生过程中的作用。目前的细胞培养模型和成骨细胞富集法可以为分析分化模式和细胞移植再生骨缺损提供大量的成骨细胞。
To enhance the understanding of differentiation patterns and bone formation capacity of hESCs, we determined the temporal pattern of osteoblastic differentiation of human embryonic stem cell-derived mesenchymal stem cells (hESCs-MSCs), the influence of a three-dimensional matrix on the osteogenic differentiation of hESCs-MSCs in long term culture and the bone forming capacity of osteoblast-like cells derived from hESCs-MSCs in calvarial defects. Incubation of hESCs-MSCs in osteogenic medium induced osteoblastic differentiation of hESCs-MSCs into mature osteoblasts in a similar chronological pattern to human bone marrow stromal cells and primary osteoblasts. Osteogenic differentiation was enhanced by culturing the cells on three-dimensional collagen scaffolds. Fluorescent activated cell sorting of alkaline phosphatase expressing cells was used to obtain an enriched osteogenic cell population for in vivo transplantation. The identification of green fluorescence protein and expression of human specific nuclear antigen in osteocytes in newly formed bone verified the role of transplanted human cells in the bone regeneration process. The current cell culture model and osteogenic cell enrichment method could provide large numbers of osteoprogenitor cells for analysis of differentiation patterns and cell transplantation to regenerate skeletal defects.
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影响因子：
15.8
作者：
Barberi T;Willis LM;Socci ND;Studer L
通讯作者：
Studer L
影响因子：
6.2
作者：
Bruder, SP;Ricalton, NS;Barry, FP
通讯作者：
Barry, FP
DOI：
10.1152/ajpheart.00781.2002
发表时间：
2003-04-01
影响因子：
4.8
作者：
Dan, P;Cheung, JCY;Moore, EDW
通讯作者：
Moore, EDW
影响因子：
4.8
作者：
Armstrong, Victoria J.;Muzylak, Mariusz;Lanyon, Lance E.
通讯作者：
Lanyon, Lance E.
DOI：
10.1159/000151746
发表时间：
2009
期刊：
Cells, tissues, organs
影响因子：
--
作者：
Brown SE;Tong W;Krebsbach PH
通讯作者：
Krebsbach PH