Functional and structural correlations of individual αIIbβ3 molecules
Functional and structural correlations of individual αIIbβ3 molecules
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DOI：
10.1182/blood-2004-04-1411
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发表时间：
2004-12-15
期刊：
影响因子：
20.3
通讯作者：
Weisel, JW
中科院分区：
文献类型：
--
作者：
Litvinov, RI;Nagaswami, C;Weisel, JW
二价阳离子Mn 2+和还原剂二硫苏糖醇直接将整联蛋白从其非活性状态转变为其活性状态。我们使用透射电子显微镜和激光镊子为基础的力谱，以确定是否结构重排诱导的这些药物在整合素β 3与其结合纤维蛋白原的能力。Mn 2+使血小板中特异性纤维蛋白原-β 3相互作用的可能性增加近20倍，Mn 2+和二硫苏糖醇都使更多的可能性专门涉及柄的远端。这些结果表明，有一个直接的相关性β 3活化和分子的整体构象。此外，它们与单个无活性和单个活性的β 1 b β 3分子与活性β 1 b β 3簇之间存在的联系平衡一致。(C)2004年，美国血液学会。
The divalent cation Mn2+ and the reducing agent dithiothreitol directly shift integrins from their inactive to their active states. We used transmission electron microscopy and laser tweezers-based force spectroscopy to determine whether structural rearrangements induced by these agents in the integrin alphallbbeta3 correlate with its ability to bind fibrinogen. Mn2+ increased the probability of specific fibrinogen-alphallbbeta3 interactions nearly 20-fold in platelets, and both Mn2+ and dithiothreitol increased the probability more tions exclusively involving the distal ends of the stalks. These results indicate that there is a direct correlation between alphallbbeta3 activation and the overall conformation of the molecule. Further, they are consistent with the presence of a linked equilibrium between single inactive and single active alphallbbeta3 molecules and active alphallbbeta3 clusters. (C) 2004 by The American Society of Hematology.