Alteration of floral organ identity in rice through ectopic expression of OsMADS16
Alteration of floral organ identity in rice through ectopic expression of OsMADS16
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DOI：
10.1007/s00425-003-1066-8
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发表时间：
2003-10-01
期刊：
影响因子：
4.3
通讯作者：
An, G
中科院分区：
文献类型：
--
作者：
Lee, S;Jeon, JS;An, G
我们采用转基因方法和酵母双杂交实验研究了水稻(Oryza sativa L.)B功能MADS-box基因，OsMADS16。获得了在玉米(Zea Mays L.)控制下异位表达OsMADS16的转基因水稻植株。泛素1启动子。显微镜观察发现，最内轮的心皮已经被雄蕊状的器官所取代，这与先前描述的拟南芥花相似。海因。突变超人以及异位表达AP3基因的超人。这些结果表明，OsMADS16在最内轮的表达诱导雄蕊发育。偶尔，心皮也会完全消失。此外，异位表达OsMADS16增强了另一种B功能基因OsMADS4的表达，导致超人表型。在酵母双杂交系统中，OsMADS16没有形成同源二聚体，而是与OsMADS4相互作用。OsMADS16还与OsMADS6和OSMADS8相互作用，这两个蛋白都与拟南芥B类和C类基因正常功能所需的SEPALLATA蛋白同源。基于基因表达模式和我们的酵母双杂交数据，我们讨论了水稻小花鳞片和雄蕊轮生中MADS结构域蛋白相互作用的四重奏模型。
We used a transgenic approach and yeast two-hybrid experiments to study the role of the rice (Oryza sativa L.) B-function MADS-box gene, OsMADS16. Transgenic rice plants were generated that ectopically expressed OsMADS16 under the control of the maize (Zea mays L.) ubiquitin1 promoter. Microscopic observations revealed that the innermost-whorl carpels had been replaced by stamen-like organs, which resembled the flowers of the previously described Arabidopsis thaliana (L.) Heynh. mutation superman as well as those ectopically expressing the AP3 gene. These results indicate that expression of OsMADS16 in the innermost whorl induces stamen development. Occasionally, carpels had completely disappeared. In addition, ectopic expression of OsMADS16 enhanced expression of OsMADS4, another B-function gene, causing superman phenotypes. In the yeast two-hybrid system, OsMADS16 did not form a homodimer but, rather, the protein interacted with OsMADS4. OsMADS16 also interacted with OsMADS6 and OSMADS8, both of which are homologous to SEPALLATA proteins required for the proper function of class-B and class-C genes in Arabidopsis. Based on the gene expression pattern and our yeast two-hybrid data, we discuss a quartet model of MADS-domain protein interactions in the lodicule and stamen whorls of rice florets.