Macronutrient-based model for dietary carbon routing in bone collagen and bioapatite
Macronutrient-based model for dietary carbon routing in bone collagen and bioapatite
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DOI：
10.1007/s12520-012-0102-7
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发表时间：
2012-12-01
影响因子：
2.2
通讯作者：
Grootes, Pieter M.
中科院分区：
文献类型：
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作者：
Fernandes, Ricardo;Nadeau, Marie-Josee;Grootes, Pieter M.
碳稳定同位素比值（δ C-13），在人体骨胶原蛋白（δ C-13（胶原））和生物磷灰石（δ C-13（生物磷灰石））中测量，是古代人类饮食重建的常用指标。潜在的假设是，人体组织广泛地反映了饮食食物来源的δ C-13信号（δ C-13（饮食））加上同位素偏移。然而，对结果的解释可能会被具有不同同位素信号（δ C-13（能量），δ C-13（蛋白质））的常量营养素（能量，即碳水化合物和脂质，以及蛋白质）的不同路径所混淆。对Froehle等人编制的对照动物饲养实验数据进行多元回归分析（考古学报37:2662-2670,2010）。我们推导了一个简单的代数常量营养素模型，其中δ C-13（生物磷灰石）=010.1+ δ C-13（日粮）（千分之一）和δ C-13（胶原）=04.8+0.74 δ C-13（蛋白质）+0.26 δ C-13（能量）（千分之一）。虽然delta C-13（生物磷灰石）的既定关系与先前已知的结果相似，但该模型还表明，delta C-13（胶原）信号的贡献源于蛋白质和能量宏量营养素惊人的一致比例。鉴于饲养实验探索了日粮宏量营养素比例的极端变化，所提出的模型及其预测的适用性在各种已知的野生动物、人类和自然环境中进行了测试。讨论了解释这些实证结果的可能生化机制。
Carbon stable isotope ratios (delta C-13), measured in human bone collagen (delta C-13(collagen)) and bioapatite (delta C-13(bioapatite)), are commonly used indicators in ancient human diet reconstruction. The underlying assumption is that human tissues broadly reflect the delta C-13 signal of dietary food sources (delta C-13(diet)) plus an isotopic offset. However, interpretation of results may be confounded by the differentiated routing of macronutrients (energy, that is carbohydrates and lipids, and protein) having associated different isotopic signals (delta C-13(energy), delta C-13(protein)). Multiple regression analyses were conducted on data from controlled animal feeding experiments compiled by Froehle et al. (J Archaeol Sci 37: 2662-2670, 2010). We derived a simple algebraic macronutrient-based model with delta C-13(bioapatite)=010.1+ delta C-13(diet) (parts per thousand) and delta C-13(collagen)=04.8+0.74 delta C-13(protein)+0.26 delta C-13(energy) (parts per thousand). While the established relationship for delta C-13(bioapatite) is similar to previously known results, the model also suggests that delta C-13(collagen) signal contributions originate from surprisingly consistent proportions of protein and energy macronutrients. Given that feeding experiments explore extreme variations in the proportion of diet macronutrients, the applicability of the proposed model and its predictions were tested in a variety of well-known, wild animal and human, natural contexts. Possible biochemical mechanisms explaining these empirical results are discussed.