Isolation and Characterization of an Aluminum-resistant Mutant in Rice.
Isolation and Characterization of an Aluminum-resistant Mutant in Rice.
复制标题
水稻耐铝突变体的分离和鉴定
DOI：
10.1186/s12284-016-0132-3
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发表时间：
2016-12
期刊：
影响因子：
--
通讯作者：
Huang CF
中科院分区：
文献类型：
--
作者：
Liu S;Gao H;Wu X;Fang Q;Chen L;Zhao FJ;Huang CF
研究背景铝毒是酸性土壤上作物生产的主要限制因素。水稻是小粒谷类作物中的高抗铝植物，但其抗铝的分子机制尚不完全清楚。本研究采用正向遗传筛选的方法，对水稻抗铝机理进行了初步研究。在这项研究中，我们筛选了一个乙基甲基砜（EMS）诱变库，分离和表征突变体的敏感性改变，铝在rice.ResultsTreatment的铝不耐籼稻品种Kasalath与20 μM铝诱导根肿胀。这一表型可以通过添加乙烯合成抑制剂氨基乙氧基乙烯基甘氨酸（AVG）来抑制，这表明乙烯的产生增加是铝胁迫下根膨胀的原因。以水稻根膨大为指标，建立了一种筛选水稻铝敏感或抗铝突变体的高效方法。通过对约5000个M2株系的筛选，鉴定出10个铝敏感突变体和1个铝抗性突变体ral 1（resistance to aluminium 1），ral 1突变体在正常生长条件下表现出短根表型，这是由于突变体细胞伸长减少所致。剂量反应实验表明，在所有铝浓度下，突变体ral 1对铝的抗性均高于野生型（WT）。突变体在酸性土壤中生长时对铝的抗性也更强。突变体在根尖（0- 1cm）的铝积累量明显低于野生型。突变体根尖细胞壁中铝含量低于野生型，而细胞液中铝含量与野生型相近。除铝外，突变体对镉的抗性也高于野生型。定量RT-PCR分析表明，已知的铝抗性基因的表达水平没有增加的突变体相比，野生型。遗传分析表明，突变体ral 1的抗铝表型受一对位于第6染色体长臂上的隐性单基因控制。通过筛选，我们发现了一个新的抗铝突变体1。ral 1对铝毒的抗性增强是由于铝与根尖细胞壁结合减少所致，其相关基因定位于第6号染色体长臂上。
BackgroundAluminum (Al) toxicity represents a major constraint for crop production on acid soils. Rice is a high Al-resistant plant species among small-grain cereals, but its molecular mechanisms of Al resistance are not fully understood. We adopted a forward genetic screen strategy to uncover the Al-resistance mechanisms in rice. In this study, we screened an ethylmethylsulfone (EMS)-mutagenized library to isolate and characterize mutants with altered sensitivity to Al in rice.ResultsTreatment of an Al-intolerant indica variety Kasalath with 20 μM Al induced root swelling. This phenotype could be suppressed by the addition of aminoethoxyvinylglycine (AVG, an ethylene synthesis inhibitor), suggesting that increased production of ethylene is responsible for the root swelling under Al stress. By utilizing the root swelling as an indicator, we developed a highly effective method to screen Al-sensitive or -resistant mutants in rice. Through screening of ~5000 M2 lines, we identified 10 Al-sensitive mutants and one Al-resistant mutantral1(resistance toaluminum 1).ral1mutant showed short root phenotype under normal growth condition, which was attributed to reduced cell elongation in the mutant. A dose-response experiment revealed thatral1mutant was more resistant to Al than wild-type (WT) at all Al concentrations tested. The mutant was also more resistant to Al when grown in an acid soil. The mutant accumulated much lower Al in the root tips (0–1 cm) than WT. The mutant contained less Al in the cell wall of root tips than WT, whereas Al concentration in the cell sap was similar between WT and the mutant. In addition to Al, the mutant was also more resistant to Cd than WT. Quantitative RT-PCR analysis showed that the expression levels of known Al-resistance genes were not increased in the mutant compared to WT. Genetic analysis indicated that the Al-resistance phenotype inral1mutant was controlled by a single recessive gene mapped on the long arm of chromosome 6.ConclusionsWe have developed a highly efficient method for the screening of rice mutants with altered Al sensitivity. We identified a novel mutantral1resistant to Al by this screening. The increased resistance ofral1to Al toxicity is caused by the reduced Al binding to the cell wall of root tips and the responsible gene is mapped on the long arm of chromosome 6.
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影响因子：
7.4
作者：
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通讯作者：
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影响因子：
7.4
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影响因子：
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作者：
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通讯作者：
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