Differential effect of temperature on histamine‐ and carbachol‐stimulated inositol phospholipid breakdown in slices of guinea‐pig cerebral cortex
Differential effect of temperature on histamine‐ and carbachol‐stimulated inositol phospholipid breakdown in slices of guinea‐pig cerebral cortex
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温度对豚鼠大脑皮层切片中组胺和卡巴胆碱刺激的肌醇磷脂分解的差异影响
DOI：
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发表时间：
1987
影响因子：
7.3
通讯作者：
J. Young
中科院分区：
文献类型：
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作者：
H. Carswell;A. Galione;J. Young
1豚鼠大脑皮层切片在37℃与[~3H]-肌醇孵育，然后在37℃或25℃暴露于组胺或卡巴胆碱，组胺刺激的[~3H]-肌醇1-磷酸([~3H]-IP1)在25℃时仅为37℃时的5~7%，而对卡巴胆碱在25℃时的反应为37℃时的45~49%。2抑制卡巴胆碱诱导的[~3H]-IP1在25℃和37℃时的亲和力相似，但25℃时对卡巴胆碱的EC50值(20±2μm)低于37℃时(42±2μm)。3组胺抑制豚鼠大脑皮层匀浆在25℃和37℃时组胺结合的IC_(50)无显著差异。4在25℃时组胺诱导的[~3H]-IP2和[~3H]-IP3积累量(以37℃时积累量的百分比表示)也明显低于相应的氨基甲胆碱。5这些观察结果表明，组胺和卡巴胆碱在激动剂诱导下形成([~3H]-IP1)的位置或途径(S)不同。
1 Slices of guinea‐pig cerebral cortex were incubated with [3H]‐inositol at 37°C before exposure to histamine or carbachol at 37°C or 25°C. Histamine‐stimulated accumulation of [3H]‐inositol 1‐phosphate ([3H]‐IP1) at 25°C was only 5–7% of that at 37°C, whereas for carbachol the response at 25°C was 45–49% of that at 37°C. 2 The affinity of benzilylcholine, obtained from inhibition of carbachol‐induced accumulation of [3H]‐IP1 was similar at 25°C and 37°C, but the EC50 for carbachol was lower at 25°C (20 ± 2 μm) than at 37°C(42 ± 2 μm). 3 The IC50 for histamine inhibition of [3H]‐mepyramine binding to homogenates of guinea‐pig cerebral cortex did not differ significantly at 25°C and 37°C. 4 Histamine‐induced accumulations of [3H]‐IP2 and [3H]‐IP3 at 25°C, expressed as a percentage of the accumulation at 37°C, were also much less than the corresponding value for carbachol. 5 These observations imply that the locus or pathway(s) of agonist‐induced formation of([3H]‐IP1) are not the same for histamine and carbachol.
凝血酶刺激的人血小板中磷酸肌醇的相互转化。
DOI：
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发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Wilson,DB;Neufeld,EJ;Majerus,PW
通讯作者：
Majerus,PW