BODIPY-Based Ratiometric Fluorescent Sensor for Highly Selective Detection of Glutathione over Cysteine and Homocysteine
BODIPY-Based Ratiometric Fluorescent Sensor for Highly Selective Detection of Glutathione over Cysteine and Homocysteine
复制标题
基于 BODIPY 的比率荧光传感器，用于高选择性检测谷胱甘肽而非半胱氨酸和同型半胱氨酸
DOI：
10.1021/ja309079f
复制
发表时间：
2012-11-21
影响因子：
15
通讯作者：
Yang, Qing-Zheng
中科院分区：
文献类型：
--
作者：
Niu, Li-Ya;Guan, Ying-Shi;Yang, Qing-Zheng
我们报道了一种基于单氯化BODIPY的比例荧光传感器，用于高选择性检测谷胱甘肽（GSH）超过半胱氨酸(Cys)/同型半胱氨酸（Hcy）。单氯化BODIPY中的氯可以通过硫代卤素亲核取代，迅速被生物硫醇的硫代酸盐所取代。Cys/Hcy而不是GSH的氨基进一步取代巯基形成氨基取代体。硫取代体和氨基取代体具有明显不同的光物理性质，因此可以区分GSH与Cys和Hcy。该传感器用于活细胞中谷胱甘肽的检测。
We report a ratiometric fluorescent sensor based on monochlorinated BODIPY for highly selective detection of glutathione (GSH) over cysteine (Cys)/homocysteine (Hcy). The chlorine of the monochlorinated BODIPY can be rapidly replaced by thiolates of biothiols through thiol halogen nucleophilic substitution. The amino groups of Cys/Hcy but not GSH further replace the thiolate to form amino-substituted BODIPY. The significantly different photophysical properties of sulfur- and amino-substituted BODIPY enable the discrimination of GSH over Cys and Hcy. The sensor was applied for detection of GSH in living cells.