A study of urinary endogenous gamma-hydroxybutyrate (GHB) levels.
A study of urinary endogenous gamma-hydroxybutyrate (GHB) levels.
复制标题
尿内源性 γ-羟基丁酸 (GHB) 水平的研究。
DOI：
10.1093/jat/27.1.40
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发表时间：
2003
影响因子：
2.5
通讯作者：
K. Reid
中科院分区：
文献类型：
--
作者：
D. Yeatman;K. Reid
近年来，伽马-羟丁酸酯(GHB)作为一种娱乐药物的使用促使法医毒理学实验室将GHB的分析纳入其常规筛查程序。羟基丁酸是人体的一种天然成分，需要在病例样本中区分内源性和外源性羟基丁酸水平，这对法医毒理学家提出了挑战。这项研究是基于对55名6岁至59岁的男性和女性自愿提供的尿样的分析，以确定人类典型的尿液内源性GHb水平。使用一种旨在将尿样中的羟丁内酯定量转化为伽马丁内酯(GBL)的水解法，对所有样品进行了初步筛选，然后进行液-液萃取，并用气相色谱-质谱仪(GC-MS)分析存在的任何GBL。作为确认试验，样品随后通过固相萃取技术提取，衍生化为GHB di-TMS，并用GC-MS进行分析。55名受试者血药浓度的中位数为1.3毫克/毫升(平均值为1.65微克/毫升，范围为0.9微克/毫升至3.5微克/毫升，标准偏差为0.68微克/毫升)。这项研究的结果证实了先前建议的常规法医分析的10微克/毫升的临界值。
In recent years, the use of gamma-hydroxybutyrate (GHB), as a recreational drug has prompted forensic toxicology laboratories to incorporate the analysis for GHB into their routine screening procedures. GHB, being a natural occurring constituent of the human body, presents a challenge for forensic toxicologists in that endogenous levels and exogenous levels of GHB need to be differentiated in case samples. This study was designed to determine typical urinary endogenous levels of GHB in humans based on the analysis of urine samples voluntarily provided by 55 male and female subjects ranging in age from 6 to 59 years. All samples were initially screened for the presence of GHB utilizing a hydrolysis method designed to quantitatively convert the GHB in urine samples to gamma-butyrolactone (GBL) followed by the liquid-liquid extraction and analysis of any GBL present by gas chromatography-mass spectrometry (GC-MS). As a confirmation test, samples were then extracted by a solid-phase extraction technique, derivatized to GHB di-TMS, and analyzed by GC-MS. The median concentration determined for the 55 subjects was 1.3 mg/mL (mean = 1.65 microg/mL, range 0.9 microg/mL to 3.5 microg/mL, standard deviation 0.68 microg/mL). The results of this study confirm the previously suggested cutoff of 10 microg/mL for routine forensic analyses.