CMC stabilized nano silver synthesis, characterization and its antibacterial and synergistic effect with broad spectrum antibiotics
CMC stabilized nano silver synthesis, characterization and its antibacterial and synergistic effect with broad spectrum antibiotics
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DOI：
10.1016/j.carbpol.2016.11.083
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发表时间：
2017-02-20
影响因子：
11.2
通讯作者：
Immanuel, G.
中科院分区：
文献类型：
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作者：
Prema, P.;Thangapandiyan, S.;Immanuel, G.
在本研究中，银纳米粒子合成还原硝酸银使用CMC水溶液，作为还原剂和封端剂。通过颜色变化观察AgNO3纳米粒子的形成，并通过紫外-可见光谱、FTIR、XRD、SEM、EDS和AFM对这些纳米粒子进行表征。观察到的FTIR峰范围为3300 - 605 cm（-1）。AFM图像清楚地显示了良好分散的纳米颗粒的表面形态。SEM图像示出了具有球形形状的纳米颗粒。通过XRD分析确保颗粒的结晶性质。纳米颗粒的抗微生物活性针对人类细菌病原体（蜡状芽孢杆菌、金黄色葡萄球菌、沙门氏菌）进行测试。表皮葡萄球菌、大肠杆菌、肺炎克雷伯氏菌、铜绿假单胞菌、鼠伤寒沙门氏菌和创伤弧菌）。纳米颗粒高度抑制细菌生长。纳米颗粒与选定的广谱抗生素组合对测试细菌的协同效应确定了强的生长抑制活性。(C)2016由Elsevier Ltd.出版
In the present study silver nanoparticles were synthesized by reduction of AgNO3 using aqueous CMC solution, which acts as both reducing and capping agent. The formation of AgNO3 nanoparticles was observed visually by color change and these nanoparticles were characterized through UV-vis spectroscopy, FTIR, XRD, SEM, EDS and AFM. The FTIR peaks observed to be ranging from 3300 to 605 cm(-1). The AFM image clearly showed the surface morphology of well dispersed nanoparticles. SEM image illustrates the nanoparticles with spherical shape. The crystalline nature of the particles was assured by XRD analysis. The antimicrobial activity of nanoparticles was tested against human bacterial pathogens (Bacillus cereus, Staphylococcus aureus, S. epidermidis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhimurium & Vibrio vulnificus). The bacterial growth was highly inhibited by the nanoparticles. The synergistic effect of nanoparticles in combination with selected broad spectrum antibiotics against the tested bacteria determined strong growth inhibitory activity. (C) 2016 Published by Elsevier Ltd.