PURIFICATION OF SENESCENCE MARKER PROTEIN-30 (SMP30) AND ITS ANDROGEN-INDEPENDENT DECREASE WITH AGE IN THE RAT-LIVER
PURIFICATION OF SENESCENCE MARKER PROTEIN-30 (SMP30) AND ITS ANDROGEN-INDEPENDENT DECREASE WITH AGE IN THE RAT-LIVER
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DOI：
10.1016/0304-4165(92)90108-7
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发表时间：
1992-04-22
期刊：
影响因子：
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通讯作者：
MARUYAMA, N
中科院分区：
文献类型：
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作者：
FUJITA, T;UCHIDA, K;MARUYAMA, N
应用新开发的二维醋酸纤维素膜电泳（2D-CAME）技术，研究了大鼠肝脏可溶性蛋白质的变化。我们检测并分离出一种新的大鼠肝脏蛋白，其含量随着年龄的增长而不依赖于雄激素。我们将这种分子量为30 kDa、pI值为4.9的蛋白质命名为衰老标志蛋白-30（SMP 30）。SMP 30的表达不受去势或去势后用丙酸睾酮处理的影响。在老年女性中也发现了与糖尿病相关的SMP 30水平降低。我们注意到另一种蛋白质的pI值为7.3，随着年龄的增长而下降。它的表达似乎是雄激素依赖性的，它是显着表达，仅在年轻和成年雄性大鼠肝脏。然后我们纯化了SMP 30并制备了抗SMP 30的抗血清。免疫组织化学分析显示，在许多检测的器官中，SMP 30的定位仅限于肝脏和肾脏。虽然肝脏中的SMP 30的量相对较大并且组织分布是特征性的，但是没有已知的蛋白质对应于该蛋白质。
Age-associated changes in the soluble proteins from rat liver were examined by a newly developed two-dimensional cellulose acetate membrane electrophoresis (2D-CAME). We detected and isolated a novel rat liver protein, the amounts of which decreased androgen-independently with aging. We designated this protein, whose molecular mass was 30 kDa and pI value was 4.9, as senescence marker protein-30 (SMP30). The expression of SMP30 was not modified by castration or treatment with testosterone propionate after castration. Age-associated decrease of SMP30 level was also recognized in aged female. We noted another protein with a pI value of 7.3 that decreased with aging. Its expression seemed to be androgen-dependent and it was markedly expressed only in young and adult male rat livers. We then purified SMP30 and prepared an anti-serum to SMP30. Immunohistochemical analysis showed that the localization of SMP30 was restricted to liver and kidney among numerous organs tested. Although the amount of SMP30 in the liver was relatively large and the tissue distribution was characteristic, no known protein corresponds to this protein.