Mechano-active scaffold design based on microporous poly(L-lactide-co-epsilon-caprolactone) for articular cartilage tissue engineering: dependence of porosity on compression force-applied mechanical behaviors.
Mechano-active scaffold design based on microporous poly(L-lactide-co-epsilon-caprolactone) for articular cartilage tissue engineering: dependence of porosity on compression force-applied mechanical behaviors.
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DOI：
10.1089/ten.2006.12.449
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发表时间：
2006-03
影响因子：
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通讯作者：
Jun Xie;Maki Ihara;Youngmee Jung;I. Kwon;S. Kim;Young Ha Kim;T. Matsuda
中科院分区：
文献类型：
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作者：
Jun Xie;Maki Ihara;Youngmee Jung;I. Kwon;S. Kim;Young Ha Kim;T. Matsuda
功能性关节软骨组织工程的一个重要组成部分是一种机械活性支架，它能响应外加的压缩应力，并且几乎不会引起永久变形。作为机械活性支架软骨组织工程系列研究的第一篇论文，本研究从生物力学的角度研究了机械活性支架材料对不同压缩方式的力学响应，以及机械活性支架材料的选择。支架由弹性微孔聚(L-丙交酯-己内酯)(PLCL)制成，具有开放的孔结构(300，约500微米)，不同的孔隙率从71%到86%不等。PLCL海绵和兔关节软骨组织分别在5kPa0.1和0.005 Hz的压力下进行压缩/卸载试验，在10、30和50%应变下进行应力松弛试验。压缩试验的加载最大应变和卸载残余应变以及应力松弛试验的最大应力和平衡应力的测量表明，孔隙率越低，其力学性能越接近天然软骨组织。在PLcL海绵中，孔隙率为71%的海绵是一种合适的软骨支架材料。
An essential component of functional articular cartilage tissue engineering is a mechano-active scaffold, which responds to applied compression stress and causes little permanent deformation. As the first paper of a series on mechano-active scaffold-based cartilage tissue engineering, this study focused on mechanical responses to various modes of loading of compression forces and subsequent selection of mechano-active scaffolds from the biomechanical viewpoint. Scaffolds made of elastomeric microporous poly(L-lactide-co-epsilon-caprolactone) (PLCL) with open-cell structured pores (300 approximately 500 microm) and with different porosities ranging from 71 to 86% were used. The PLCL sponges and rabbit articular cartilage tissue were subjected to compression/unloading tests (0.1 and 0.005 Hz) at 5 kPa, and stress relaxation tests at 10, 30, and 50% strain. The measurements of the maximum strain under loading and residual strain under unloading for compression tests and the maximum stress and equilibrium stress in the stress relaxation test showed that the lower the porosity, the closer the mechanical properties are to those of native cartilage tissue. Among the PLCL sponges, the sponge with 71% porosity appears to be a suitable cartilage scaffold.