Absorption and metabolism of cyanidin-3-glucoside and cyanidin-3-rutinoside extracted from wild mulberry (Morus nigra L.) in rats
Absorption and metabolism of cyanidin-3-glucoside and cyanidin-3-rutinoside extracted from wild mulberry (Morus nigra L.) in rats
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DOI：
10.1016/j.nutres.2007.12.012
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发表时间：
2008-03-01
影响因子：
4.5
通讯作者：
Lajolo, Franco Maria
中科院分区：
文献类型：
--
作者：
Hassimotto, Neuza Mariko Ayrnoto;Genovese, Maria Ines;Lajolo, Franco Maria
从肠道中摄取食物中的花青素的机制(以及花色苷的类型)尚不清楚。用Wistar大鼠灌胃给予野桑花青素提取物，其主要成分为花青素-3-葡萄糖苷(79%)和花青素-3-芦丁苷(Cy-3-RUT)(19%)，并测定其在血浆、肾脏和胃肠道的浓度。这两种糖基化形式在口服后15分钟出现最大浓度，在血浆和肾脏中都是如此。血浆中的氰基-3-葡萄糖苷和Cy-3-RUT以葡萄糖醛酸苷、氰基硫酸盐和不变形式存在。血药浓度-时间曲线下面积(AUC(0-8h))分别为2.76+/-0.88微克/小时/毫升和9.74+/-0.75微克/小时/克。尽管吸收率低，但血浆花色苷水平的增加导致了抗氧化能力的显著增加(P<；0.05)。在胃肠道(胃、小肠和大肠)，氰基糖苷没有变化，但存在少量的苷元形式。给药8小时后，胃肠道中不再检测到花色苷苷。体外发酵结果表明，2种花青素糖苷均被大鼠结肠菌群完全代谢，解释了它们消失的原因。此外，它们的两种降解产物--花青素和原儿茶酸在血浆中未检测到，可能不影响血浆抗氧化能力。根据外翻囊模型发现，花青素通过钠依赖的葡萄糖转运蛋白跨肠细胞运输。(C)2008 Elsevier Inc.保留所有权利。
The mechanism of uptake of anthocyanins (as well as the type) from food in the intestine is not clear. Anthocyanin-rich extract from wild mulberry, composed of cyanidin-3-glucoside (79%) and cyanidin-3-rutino side (cy-3-rut) (19%), was orally administered to Wistar rats, and their concentrations were determined in plasma, kidney, and the gastrointestinal (GI) tract. The 2 glycosylated forms showed maximum concentration at 15 minutes after oral administration, both in plasma and kidney. The cyanidin-3-glucoside and cy-3-rut were found in plasma as glucuronides, as sulfates of cyanidin, and as unchanged forms. The area under the curve of concentration vs time (AUC(0-8h)) was 2.76 +/- 0.88 mu g hour/mL and 9.74 +/- 0.75 mu g hour/g for plasma and kidney, respectively. In spite of the low absorption, the increase in plasma anthocyanin level resulted in a significant increase in antioxidant capacity (P < .05). In the GI tract (stomach and small and large intestines), cyanidin glycosides were found unchanged, but a low amount of the aglycone form was present. Anthocyanin glycosides were no longer detected in the GI tract after 8 hours of administration. In vitro fermentation showed that the 2 cyanidin glycosides were totally metabolized by the rat colonic microflora, explaining their disappearance. In addition, the 2 products of their degradation, cyanidin and protocatechuic acid, were not detected in plasma and probably do not influence plasma antioxidant capacity. As found by the everted sac model, anthocyanins were transported across the enterocyte by the sodium-dependent glucose transporter. (c) 2008 Elsevier Inc. All rights reserved.