Sensitive circular dichroism spectroscopy based on nonlinear degenerate four-wave mixing.
Sensitive circular dichroism spectroscopy based on nonlinear degenerate four-wave mixing.
复制标题
基于非线性简并四波混频的灵敏圆二色光谱。
DOI：
10.1021/ac00069a009
复制
发表时间：
1993
影响因子：
7.4
通讯作者：
Tong,WG
中科院分区：
文献类型：
--
作者：
Nunes,JA;Tong,WG
简并四波混频是测量圆二向色性的一种有效而灵敏的激光分析光谱方法。利用前向散射简并四波混频光学装置，可以获得简单的光学对准、高效的混频和非常有效地利用低激光功率。这种基于非线性激光的圆二色谱方法具有许多优点，包括容易和高效的光学信号采集，使用非常短的分析物路径长度(例如0.1 mm)，以及可与传统的基于激光或非激光的圆二色谱方法相媲美或更好的卓越检测灵敏度。在分析物路径长度仅为0.1 mm，探针体积为98pl的情况下，对(+)Co(En)33+的圆二色谱质量检测下限为0.68pg或2.8fmol。
Degenerate four-wave mixing is demonstrated as an effective and sensitive laser analytical spec-troscopic method for circular dichroism measure-ments. A forward-scattering degenerate four-wave mixing optical setup is used to obtain simple optical alignment, highly efficient wave mixing, and very effective use of low laser power. This nonlinear laser-based circular dichroism method offers many advantages, including easy and efficient optical signal collection, use of very short analyte path lengths (eg, 0.1 mm), and excellent detection sensitivity that is comparable or better than conventional laser-based or non-laser-based circular dichroism methods. Using an analyte path length of only 0.1 mm, and a probe volume of 98 pL, a circular dichroism mass detection limit of 0.68 pg or 2.8 fmol is reported for (+) Co (en) 33+.