Characteristics of a Pink‐Pigmented Bacterium Isolated from Biofilm in a Cooling Tower in Tokyo, Japan
Characteristics of a Pink‐Pigmented Bacterium Isolated from Biofilm in a Cooling Tower in Tokyo, Japan
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从日本东京冷却塔生物膜中分离出的粉红色细菌的特征
DOI：
10.1111/j.1348-0421.2007.tb03951.x
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发表时间：
2007
影响因子：
2.6
通讯作者：
M. Fukuyama
中科院分区：
文献类型：
--
作者：
K. Furuhata;K. Goto;Y. Kato;Keiko Saitou;J. Sugiyama;M. Hara;S. Yoshida;M. Fukuyama
菌株K‐20是从日本东京冷却塔的生物膜中分离出来的一种革兰氏阴性、非运动、非孢子形成和严格的好氧棒，它产生淡粉色色素。采用表型试验和系统发育分析对该菌株的分类特征进行了研究。16S rRNA基因序列的系统发育分析表明该菌株与吉拉氏玫瑰单胞菌亚种有关。玫瑰，玫瑰单胞菌吉拉蒂亚种。gilardii，宫颈玫瑰单胞菌和粘膜玫瑰单胞菌序列相似度为94.3-94.6。25-40℃、pH 5.0-10.0条件下生长最佳，35℃、pH 7.0条件下生长最佳。在≧2% NaCl的条件下不发生生长。API 20NE鉴定系统对脲酶、L -阿拉伯糖、葡萄糖酸钾、己二酸、苹果酸和柠檬酸三钠（API代码0201465）检测结果为阳性。菌株K‐20的优势脂肪酸为C18:1Δ11（50.8%）和C16:1（17.2%）。细胞中醌类成分主要为泛醌10 (Q‐10)，G+C含量为72.0 mol%。根据表型、化学分类和系统发育数据，我们认为菌株K‐20 （=JCM 14634）是玫瑰单胞菌属的一个新种。
Strain K‐20, a Gram‐negative, non‐motile, non‐spore‐forming and strictly aerobic rod, which produces a pale pink pigment, was isolated from biofilm in a cooling tower in Tokyo, Japan. The taxonomic feature of the strain was studied using phenotypic tests and phylogenetic analysis. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain was related to Roseomonas gilardii subsp. rosea, Roseomonas gilardii subsp. gilardii, Roseomonas cervicalis and Roseomonas mucosa at 94.3–94.6 sequence similarities. Growth occurred at 25–40 C and pH 5.0–10.0, optimal at 35 C and pH 7.0. Growth did not occur in the presence of ≧2% NaCl. The API 20NE identification system gave a positive result for urease, L‐arabinose, potassium gluconate, adipic acid, malic acid and trisodium citrate (API code number 0201465). The predominant fatty acids of strain K‐20 were C18:1Δ11 (50.8%) and C16:1 (17.2%). Cells contained ubiquinone 10 (Q‐10) as the major quinone and the G+C content was 72.0 mol%. Based on phenotypic, chemotaxonomic and phylogenetic data, it was assumed that strain K‐20 (=JCM 14634) is a novel species of the genus Roseomonas.