Aflatoxin B1-induced changes of glutathione-S-transferase activity in the plasma and liver of the rat.
Aflatoxin B1-induced changes of glutathione-S-transferase activity in the plasma and liver of the rat.
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DOI：
10.2298/vsp0304415s
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发表时间：
2003-07-01
影响因子：
0.2
通讯作者：
Krtolica, Koviljka V
中科院分区：
文献类型：
--
作者：
Strelic, Natasa J;Saicic, Zorica S;Krtolica, Koviljka V
背景技术背景：本文研究了低剂量黄曲霉毒素B_1（AFB_1）和肝部分切除（PH）对大鼠血浆和肝脏谷胱甘肽-S-转移酶（GST）活性的影响。第一组（I）和第二组（II）分别用新鲜溶解在二甲基亚砜（DMSO）中的AFB 1处理，并在大鼠经历假手术或40%PH后24小时以50 μ g/只的剂量单次腹腔内给药。第三组（III）动物在8周期间每周用1 mg AFB 1 -5天的总剂量治疗。结果：与对照组相比，我们观察到所有实验组的血浆中GST活性显著增加（p < 0.02至p < 0.005）。结论：AFB 1单次和多次给药均可引起大鼠血浆和肝脏GST活性的长期升高，肝部分切除对这一现象无明显影响。
BACKGROUND: The influence of low doses of aflatoxin B1(AFB1) and partial hepatectomy (PH) on glutathione-S-transferase (GST) activity was studied in the plasma and liver of the rat.METHODS: The animals were divided into four groups. The first (I) and the second (II) group were treated with AFB1 freshly dissolved in dimethyl sulphoxide (DMSO), and administered as a single intraperitoneal dose of 50 micrograms/rat 24 hrs after the rats had undergone either sham operation or, 40% PH, respectively. The third group (III) of animals was treated with a total dose of 1 mg AFB1-5 days per week during a period of 8 weeks. The non-treated animals were used as controls (C).RESULTS: We observed a significant increase of GST activity in the plasma of all experimental groups compared to the controls (C), (p < 0.02 to p < 0.005). In the liver, the GST activity of all experimental groups was also significantly increased, compared to the controls (from p < 0.02 to p < 0.005).CONCLUSION: The administration of both single and multiple doses of AFB1 led to long term increase of GST activity in the rat plasma and liver, and partial hepatectomy had no significant effect on this phenomenon.