Comparative biodistribution studies of technetium-99m radiolabeled amphiphilic nanoparticles using three different reducing agents during the labeling procedure
Comparative biodistribution studies of technetium-99m radiolabeled amphiphilic nanoparticles using three different reducing agents during the labeling procedure
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DOI：
10.1002/jlcr.3097
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发表时间：
2013-12-01
影响因子：
1.8
通讯作者：
Goracinova, Katerina
中科院分区：
文献类型：
--
作者：
Geskovski, Nikola;Kuzmanovska, Sonja;Goracinova, Katerina
考虑到文献中令人困惑的生物分布数据和很少报道的警报以及我们初步的生物分布结果，我们决定评估在直接氯化亚锡Tc-99m标记过程中常见的Tc-99m（锝-99m）-氧化锡胶体的相互作用和干扰，并评估其对两亲性聚（乳酸-羟基乙酸）纳米颗粒生物分布模式的影响。为了证实我们的论点，除了氯化亚锡，我们还使用了两种不同的不形成胶体颗粒的还原剂。硼氢化钠的使用在以前的文献中有报道，而二亚硫酸钠是第一次用于纳米颗粒的Tc-99m直接标记程序。我们论文的结果清楚地区分了有或没有胶体杂质的样品，这些样品来自于标签程序，并对放射化学、物理化学评估和生物分布研究进行了逻辑跟进，澄清了先前报道的氧化锡胶体干扰数据。以二亚硫酸钠作为还原剂标记的tc -99m纳米颗粒复合物显示出适当的标记效果、稳定性和在生物分布研究中进一步应用的潜力，从而为使用硼氢化钠时的低络合物稳定性问题和使用二亚硫酸钠的胶体氧化锡干扰问题提供了解决方案。版权所有：John Wiley & Sons， Ltd。
Considering the confusing biodistribution data through the literature and few reported alerts as well as our preliminary biodistribution results, we decided to evaluate the interaction and interference of the commonly present Tc-99m (technetium-99m)-stannic oxide colloid during the direct stannous chloride Tc-99m-labeling procedure and to assess its influence on the biodistribution pattern of amphiphilic poly(lactic-co-glycolic acid) nanoparticles. In order to confirm our thesis, beside stannous chloride, we employed two different reducing agents that don't form colloidal particles. The use of sodium borohydride was previously reported in the literature, whereas sodium dithionite was adapted for the first time in the Tc-99m direct labeling procedure for nanoparticles. The results in our paper clearly differentiate among samples with and without colloidal impurities originating from the labeling procedure with a logical follow up of the radiochemical, physicochemical evaluation, and biodistribution studies clarifying previously reported data on stannic oxide colloidal interference. Tc-99m-nanoparticle complex labeled with sodium dithionite as reducing agent illustrated appropriate labeling efficacy, stability, and potential for further use in biodistribution studies thus providing solution for the problem of low-complex stability when sodium borohydride is used and colloidal stannic oxide interference for stannous chloride procedure. Copyright (c) 2013 John Wiley & Sons, Ltd.