The effects of graphene quantum dots on the maturation of mouse oocytes and development of offspring
The effects of graphene quantum dots on the maturation of mouse oocytes and development of offspring
复制标题
石墨烯量子点对小鼠卵母细胞成熟及后代发育的影响
DOI：
10.1002/jcp.28062
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发表时间：
2019
影响因子：
5.6
通讯作者：
Wang Hai-Long
中科院分区：
文献类型：
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作者：
Lin Yan-Hong;Zhuang Shu-Xin;Wang Ya-Long;Lin Sheng;Hong Zi-Wei;Liu Yu;Xu Lin;Li Fei-Ping;Xu Bai-Hui;Chen Ming-Huang;He Shu-Wen;Liao Bao-Qiong;Fu Xian-Pei;Jiang Zhong-Qing;Wang Hai-Long
近年来，石墨烯纳米材料因其优异的力学、热学、化学、光学等性能以及良好的生物相容性，特别是在生物医学方面的应用，引起了人们的极大关注。然而，人们担心纳米材料的独特特性可能会产生不良影响。因此，在本研究中，我们试图通过体外和体内研究系统地研究石墨烯量子点（GQD）对小鼠卵母细胞成熟和后代发育的影响。在体外实验中，我们发现高剂量组（1.0-1.5 mg/ml）2的第一极体挤出率显著降低，观察到GQDs暴露后纺锤体迁移和肌动蛋白帽形成的失败。其机制可能与活性氧积累和DNA损伤有关。此外，透射电子显微镜研究表明，GQD可能已经内化到卵母细胞中，倾向于在细胞核中积累，并严重影响线粒体形态，包括肿胀和空泡化的线粒体，伴随着嵴改变，致密的线粒体基质量减少。在体内，当妊娠小鼠在妊娠8.5天（GD，8.5）暴露于GQD时，我们发现高剂量的GQD暴露（30 mg/kg）显著影响平均胎儿长度;然而，所有第二代雌性小鼠均正常生长，达到性成熟，并在与健康雄性小鼠交配后产下健康后代。在这项研究中提出的结果是重要的GQD的生物医学应用的未来调查。
Recently, graphene nanomaterials have attracted tremendous attention and have been utilized in various fields because of their excellent mechanical, thermal, chemical, optical properties, and good biocompatibility, especially in biomedical aspects. However, there is a concern that the unique characteristics of nanomaterials may have undesirable effects. Therefore, in this study, we sought to systematically investigate the effects of graphene quantum dots (GQDs) on the maturation of mouse oocytes and development of the offspring via in vitro and in vivo studies. In vitro, we found that the first polar body extrusion rate in the high dosage exposure groups (1.0–1.5 mg/ml) 2 decreased significantly and the failure of spindle migration and actin cap formation after GQDs exposure was observed. The underlying mechanisms might be associated with reactive oxygen species accumulation and DNA damage. Moreover, transmission electron microscope studies showed that GQDs may have been internalized into oocytes, tending to accumulate in the nucleus and severely affecting mitochondrial morphology, which included swollen and vacuolated mitochondria accompanied by cristae alteration with a lower amount of dense mitochondrial matrix. In vivo, when pregnant mice were exposed to GQDs at 8.5 days of gestation (GD, 8.5), we found that high dosage of GQD exposure (30 mg/kg) significantly affected mean fetal length; however, all the second generation of female mice grew up normal, attained sexual maturity, and gave birth to a healthy offspring after mating with a healthy male mouse. The results presented in this study are important for the future investigation of GQDs for the biomedical applications.