MAJOR DETERMINANTS OF THE SPECIFICITY OF INTERACTION BETWEEN SMALL NUCLEAR RIBONUCLEOPROTEIN-U1A AND RIBONUCLEOPROTEIN-U2B'' AND THEIR COGNATE RNAS
MAJOR DETERMINANTS OF THE SPECIFICITY OF INTERACTION BETWEEN SMALL NUCLEAR RIBONUCLEOPROTEIN-U1A AND RIBONUCLEOPROTEIN-U2B'' AND THEIR COGNATE RNAS
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DOI：
10.1038/345502a0
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发表时间：
1990-06-07
期刊：
影响因子：
64.8
通讯作者：
MATTAJ, IW
中科院分区：
文献类型：
--
作者：
SCHERLY, D;BOELENS, W;MATTAJ, IW
已经检查了U1和U2小核(sn)RNA及其同源结合蛋白U1A和U2B”相互作用的特异性的基础。U1A蛋白自身识别U1 snRNA，而U2B”仅在第二种蛋白U2A'存在时特异性结合U2 snRNA。两个 RNA 之间两个核苷酸的交换或两个蛋白质之间八个氨基酸的交换会逆转结合特异性。
The basis of the specificity of interaction of Ul and U2 small nuclear (sn)RNAs and their cognate binding proteins, U1A and U2B", has been examined. The U1A protein recognizes Ul snRNA on its own, whereas U2B" binds specifically to U2 snRNA only in the presence of a second protein, U2A'. Exchange of two nucleotides between the two RNAs or of eight amino acids between the two proteins reverses binding specificity.