A strikingly constant ratio exists between Langerhans cells and other epidermal cells in human skin.: A stereologic study using the optical disector method and the confocal laser scanning microscope
A strikingly constant ratio exists between Langerhans cells and other epidermal cells in human skin.: A stereologic study using the optical disector method and the confocal laser scanning microscope
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DOI：
10.1046/j.1523-1747.2001.01247.x
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发表时间：
2001-02-01
影响因子：
6.5
通讯作者：
Fartasch, M
中科院分区：
文献类型：
--
作者：
Bauer, J;Bahmer, FA;Fartasch, M
朗格汉斯细胞在人体表皮的免疫监视中起着重要作用。因此，可以预期与其他表皮细胞有一定的分布和数量关系。为了对表皮朗格汉斯细胞群体进行量化，人们使用二维定量方法对垂直切片或表皮片材进行了广泛的研究。由于取样程序、树突形状和超基底、非随机分布的朗格汉斯细胞，使用垂直切片的方法相当复杂，而表皮片剂在朗格汉斯细胞与总表皮细胞的数量关系和表皮形态方面有其局限性。为了提高数据的准确性，应用三维解剖结合激光共聚焦扫描显微镜对8例女性正常乳腺组织24例冰冻切片单位体积的朗格汉斯细胞和其他表皮细胞核进行了定量。此外，还计算了朗格汉斯细胞与其他表皮细胞的比率、每次活检的细胞数量和每一皮肤表面积。为了通过收缩最大限度地减少偏差，参考体积用Cavalieri原理估算。在乳房皮肤中，1个朗格汉斯细胞与53个其他表皮细胞的比例是恒定的(个体间相关系数：0.952，p
Langerhans cells play an important part in the immune surveillance of the human epidermis. Therefore, a certain distribution and numerical relationship to other epidermal cells can be expected. To quantify epidermal Langerhans cells population extensive studies have been performed using two-dimensional quantification methods on vertical sections or epidermal sheet preparations. Whereas methods using vertical sections were complicated considerably by the sampling procedure, the dendritic shape, and the suprabasal, nonrandom distribution of Langerhans cells, epidermal sheet preparations have their limitations regarding the numerical relationship of Langerhans cells to total epidermal cells and the epidermal morphology as such. In order to improve the validity of data the three-dimensional dissector method combined with confocal laser scanning microscopy has been applied to quantify the number of langerhans cells and other epidermal cell nuclei per volume unit in cryosections of 24 punch biopsies of normal breast skin of eight women. Furthermore, the ratio of Langerhans cells to other epidermal cells, their number per biopsy, and per skin surface area were calculated. To minimize the bias by shrinkage the reference volume was estimated using Cavalieri's principle. A constant ratio of one Langerhans cells to 53 other epidermal cells was identified in breast skin (interindividual correlation coefficient: 0.952, p