Immunoelectrophoretic characterization of the ADP/ATP carrier from heart, kidney, and liver.
Immunoelectrophoretic characterization of the ADP/ATP carrier from heart, kidney, and liver.
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心脏、肾脏和肝脏 ADP/ATP 载体的免疫电泳表征。
DOI：
10.1016/0003-9861(85)90836-7
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发表时间：
1985
影响因子：
3.9
通讯作者：
M. Klingenberg
中科院分区：
文献类型：
--
作者：
H. Schultheiss;M. Klingenberg
早期研究表明 ADP ATP 载体具有器官特异性。我们使用改良的电荷转移交叉免疫电泳来更精确地表征这种去污剂溶解的疏水膜蛋白。来自心脏、肾脏和肝脏的载体蛋白之间的免疫学差异通过第一维中三种配体-蛋白质复合物的不同电泳迁移以及第二维中沉淀物的不同染色强度、清晰度和形状来证明。然而，针对心脏和肾脏蛋白的抗体显示出三种抗原之间的交叉反应性。结果与 ADP ATP 载体具有器官特异性抗原决定簇的观点一致，尽管来自心脏、肾脏和肝脏的载体蛋白之间存在部分同一性。
Earlier studies gave an indication for an organ specificity of the ADP ATP carrier. We used a modified charge-shift crossed immunoelectrophoresis for a more precise immunochemical characterization of this detergent-solubilized hydrophobic membrane protein. Immunological differences between the carrier protein from heart, kidney, and liver were demonstrated by a different electrophoretic migration of the three ligand-protein complexes in the first dimension and a distinct staining intensity, sharpness, and shape of the precipitates in the second dimension. However, the antibodies against the heart and kidney protein showed a cross-reactivity between the three antigens. The results are consistent with the view that the ADP ATP carrier has organ-specific antigenic determinants although there is a partial identity between the carrier proteins from heart, kidney, and liver.