A and H blood group antigens as markers of sucrase-isomaltase from the enterocyte-like differentiated human colon carcinoma cell lines HT-29 and Caco-2.
A and H blood group antigens as markers of sucrase-isomaltase from the enterocyte-like differentiated human colon carcinoma cell lines HT-29 and Caco-2.
复制标题
A 和 H 血型抗原作为来自肠细胞样分化人结肠癌细胞系 HT-29 和 Caco-2 的蔗糖酶-异麦芽糖酶标记。
作者：
I. Chantret;G. Chevalier;E. Dussaulx;A. Zweibaum
本工作的目的是研究肠上皮样分化的人结肠癌细胞株的蔗糖酶-异麦芽糖酶是否携带ABH系统的血型抗原。对6株A型血型(HT-29、SW-480、Co-115)或O型血型(Caco-2、HRT-18、HCT-8R)的细胞培养株进行了研究。只有在无葡萄糖条件下生长的HT-29细胞(HT-29 Glc-)和Caco-2细胞表现出肠上皮细胞分化，其顶端表面存在蔗糖酶-异麦芽糖酶。抗-A抗体与HT-29GLC-和UEA-I与Caco-2细胞的结合呈现与抗蔗糖酶-异麦芽糖酶抗体相同的顶端免疫荧光染色模式，而在未分化的细胞中仅观察到膜结合。在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法和免疫印迹法中，HT-29细胞和Caco-2细胞的蔗糖酶-异麦芽糖酶分别与抗A抗体和UEA-I反应。用UEA-I或抗-A抗体免疫沉淀同一细胞的毛刷状富集组分，对Caco-2细胞和UEA-I的蔗糖酶活性的抑制率分别达到80%和50%。在相应的裸鼠肿瘤中也得到了类似的结果：抗A抗体在HT-29和UEA-I在Caco-2肿瘤中与抗蔗糖酶异麦芽糖酶抗体结合在相同的顶端结构；蔗糖酶异麦芽糖酶免疫从肿瘤结合的抗A抗体(HT-29)或UEA-I(Caco-2)中分离出来。这些结果支持这样的假设，即来自肠细胞样分化的人结肠癌细胞的蔗糖酶异麦芽糖酶携带ABH系统的血型抗原。这些发现表明，已经显示ABH抗原表达的顶端模式的结肠癌应该筛查其可能的肠细胞分化。
The purpose of this work was to investigate whether sucrase-isomaltase from enterocyte-like differentiated human colon carcinoma cell lines carries blood group antigens of the ABH system. Six cultured lines of blood group A (HT-29, SW-480, Co-115) or O phenotype (Caco-2, HRT-18, HCT-8R) were studied. Only HT-29 cells grown in the absence of glucose (HT-29 Glc-) and Caco-2 cells express an enterocytic differentiation with the presence of sucrase-isomaltase on the apical surface of the cells. Binding of anti-A antibodies to HT-29 Glc- and of UEA-I to Caco-2 cells gave the same apical immunofluorescence pattern of staining as did anti-sucrase-isomaltase antibodies, whereas only a membrane binding was observed in nondifferentiated cells. Sucrase-isomaltase immunoisolated from HT-29 Glc- and Caco-2 cells reacted with anti-A antibodies and Ulex europaeus agglutinin-I (UEA-I), respectively, at sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot. Immunoprecipitation of solubilized brush border-enriched fractions from the same cells with UEA-I or anti-A antibodies resulted in an inhibition of sucrase activity which reached congruent to 80% for Caco-2 cells with UEA-I and approximately equal to 50% for HT-29 cells with anti-A antibodies. Similar results were obtained in the corresponding tumors in nude mice: anti-A antibodies in HT-29 and UEA-I in Caco-2 tumors bound to the same apical structures as did anti-sucrase-isomaltase antibodies; sucrase-isomaltase immunoisolated from the tumors bound anti-A antibodies (HT-29) or UEA-I (Caco-2). These results support the hypothesis that sucrase-isomaltase from enterocyte-like differentiated human colon cancer cells carries blood group antigens of the ABH system. These findings suggest that colon cancers which have been shown to display an apical pattern of expression of ABH antigens should be screened for their possible enterocytic differentiation.
A 和 B 同种抗原在正常和恶性固定人体组织中的单克隆抗体定位。
DOI：
--
发表时间：
1984
期刊：
The American journal of pathology
影响因子：
--
作者：
Ernst,C;Thurin,J;Atkinson,B;Wurzel,H;Herlyn,M;Stromberg,N;Civin,C;Koprowski,H
通讯作者：
Koprowski,H