Hemostatic properties of glucosamine-based materials
Hemostatic properties of glucosamine-based materials
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DOI：
10.1002/jbm.a.30877
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发表时间：
2007-01-01
影响因子：
4.9
通讯作者：
Vournakis, John N.
中科院分区：
文献类型：
--
作者：
Fischer, Thomas H.;Bode, Arthur P.;Vournakis, John N.
含葡糖胺和N-乙酰葡糖胺的聚合物正被用于越来越多的生物医学应用，包括用于表面（局部）止血的产品。本文介绍的研究调查了基于葡糖胺的材料的结构（构象）和功能（止血激活）之间的关系。研究了几种聚合物系统，包括从含有聚-N-乙酰基葡糖胺聚合物的微藻源分离的纤维，所述聚-N-乙酰基葡糖胺聚合物以平行氢键合的三级结构组织并且可以被化学改性为反平行取向;以及微藻纤维的凝胶制剂衍生物，其由部分脱乙酰基（F2凝胶）和完全脱乙酰基（F3凝胶）聚合物组成。比较了聚-N-乙酰氨基葡萄糖纤维衍生材料与甲壳素、壳聚糖和商业壳聚糖基产品的性能。对基于葡糖胺的材料进行了几项研究，包括（1）分析材料激活血小板和内源性凝血级联转换的能力，（2）通过血栓弹性描记术检查富血小板血浆和基于葡糖胺的材料的混合物的粘弹性，以及（3）扫描电子显微镜研究，以检查基于葡糖胺的材料的形态。所示结果表明，对所研究的基于葡糖胺的材料的止血反应高度依赖于其化学性质和三级/四级结构。发现独特的天然微藻纤维与所研究的其他材料相比具有强烈的促止血活性。(c)2006 Wiley Periodicals，Inc.
Glucosamine- and N-acetyl glucosamine-containing polymers are being used in an increasing number of biomedical applications, including in products for surface (topical) hemostasis. The studies presented here investigate the relationship between the structure (conformation) and function (activation of hemostasis) of glucosamine-based materials. Several polymer systems were studied, including fibers isolated from a microalgal source containing poly-N-acetyl glucosamine polymers that are organized in a parallel, hydrogen-bonded tertiary structure and can be chemically modified to an antiparallel orientation; and gel formulation derivatives of the microalgal fibers consisting of partially deacetylated (F2 gel) and fully deacetylated (F3 gel) polymers. Comparison of the properties of the poly-N-acetyl glucosamine fiber-derived materials with chitin, chitosan, and commercial chitosan-based products are presented. Several studies were performed with the glucosamine-based materials, including (1) an analysis of the ability of materials to activate platelets and turnover of the intrinsic coagulation cascade, (2) an examination of the viscoelastic properties of mixtures of platelet-rich plasma and the glucosamine-based materials via thromboelastography, and (3) scanning electron microscopic studies to examine the morphology of the glucosamine-based materials. The results presented demonstrate that hemostatic responses to the glucosamine-based materials studied are highly dependent on their chemical nature and tertiary/quaternary structure. The unique natural microalgal fibers were found to have strongly prohemostatic activity compared to the other materials studied. (c) 2006 Wiley Periodicals, Inc.