Neuroprotective effect of latanoprost on rat retinal ganglion cells
Neuroprotective effect of latanoprost on rat retinal ganglion cells
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DOI：
10.1007/s00417-005-0215-0
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发表时间：
2006-01
期刊：
影响因子：
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通讯作者：
H. Kudo;T. Nakazawa;M. Shimura;Hidetoshi Takahashi;N. Fuse;K. Kashiwagi;M. Tamai
中科院分区：
文献类型：
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作者：
H. Kudo;T. Nakazawa;M. Shimura;Hidetoshi Takahashi;N. Fuse;K. Kashiwagi;M. Tamai
目的探讨玻璃体内注射拉坦前列素（latanoprost）对N-甲基-D-天冬氨酸（N-methyl-D-aspartic acid，NMDA）或视神经切断所致视网膜神经节细胞（retinal ganglion cell，RGC）损伤的保护作用。在NMDA注射或视神经轴突切断术之前，玻璃体内给予0.03、0.3、3、30和300 pmol剂量的拉坦前列素。结果NMDA损伤后7天，与对照组（556±122 cells/mm 2）相比，阿坦前列素剂量大于30 pmol时，存活的RGCs数量显著增加（846±178 cells/mm 2，P =0.0166）。结论玻璃体内注射拉坦前列素对NMDA或视神经切断引起的大鼠RGC损伤具有神经保护作用，但其药理学特征不同。
BackgroundTo investigate the neuroprotective effect of intravitreal administration of latanoprost on retinal ganglion cell (RGC) damage induced by N-methyl-D-aspartic acid (NMDA) or optic nerve axotomy.MethodsUsing Sprague-Dawley rats, retinal ganglion cell damage was induced by either intravitreal administration of NMDA or optic nerve axotomy. Latanoprost at doses of 0.03, 0.3, 3, 30 and 300 pmol was administered intravitreally before NMDA injection or optic nerve axotomy. Retinal damage was evaluated by counting the number of surviving RGCs retrogradely labeled with fluorogold under the microscope.ResultsSeven days after the NMDA injury, the number of surviving RGCs was significantly increased at doses of more than 30 pmol atanoprost (846±178 cells/mm2P=0.0166) compared with vehicle control (556±122 cells/mm2). Ten days after the optic nerve axotomy, the number of surviving RGC was significantly increased even at a dose of 0.3 pmol (815±239 cells/mm2,P=0.0359) compared with control (462±75 cells/mm2).ConclusionsIntravitreal administration of latanoprost has a neuroprotective effect on rat RGC damage induced by either NMDA or optic nerve axotomy, while its pharmacological features are different.