Atrial natriuretic peptide attenuates kidney-lung crosstalk in kidney injury
Atrial natriuretic peptide attenuates kidney-lung crosstalk in kidney injury
复制标题
DOI：
10.1016/j.jss.2013.07.033
复制
发表时间：
2014-01-01
影响因子：
2.2
通讯作者：
Tomita, Makoto
中科院分区：
文献类型：
--
作者：
Tulafu, Miniwan;Mitaka, Chieko;Tomita, Makoto
背景：肾缺血再灌注损伤(IRI)是心血管手术后急性肾损伤的常见原因，继而加重氧合作用。心钠素(ANP)具有利钠、利尿、抗炎等作用。材料与方法：将大鼠麻醉、气管切开、机械通气，随机分为4组：生理盐水+再灌注组(n=12)、心钠素+再灌注组(n=12)、心钠素+假手术组(n=6)、生理盐水+假手术组(n=6)。分别于夹闭前5min开始注射生理盐水(6mL/kg/h)或心钠素(0.2mU/kg/min)，注射速度为6mL/kg/h。夹闭左侧肾蒂30min，造成肾IRI。分别于术前、术后1、2、3h测定血流动力学、动脉血气、血肌酐和乳酸浓度。测定肺湿干比。结果：肾IRI可引起代谢性酸中毒、肺水肿、血浆肌酐和乳酸浓度升高，肾和肺组织细胞因子表达和组织学定位增加。ANP可预防IRI所致的代谢性酸中毒、肺水肿、内生肌酸、乳酸和细胞因子mRNA的表达增加，减轻肾和肺组织中肿瘤坏死因子-α的组织学定位，增加氧合作用。结论：ANP对肾IRI大鼠的肾脏和肺具有保护和抗炎作用，提示ANP可减轻肾-肺串扰。(C)2014 Elsevier Inc.保留所有权利。
Background: Renal ischemia-reperfusion injury (IRI) is a common cause of acute kidney injury after cardiovascular surgery, which in turn deteriorates oxygenation. Atrial natriuretic peptide (ANP) has natriuretic, diuretic, and anti-inflammatory effects. To elucidate whether renal IRI induces inflammation in the kidney and lung and ANP attenuates kidney-lung crosstalk.Materials and methods: The rats were anesthetized, tracheostomized, mechanically ventilated, and randomized to four groups: saline + IRI (n = 12), ANP + IRI (n = 12), ANP + sham (n = 6), and saline + sham (n = 6). Saline (6 mL/kg/h) or ANP (0.2 mu g/kg/min) at the rate of 6 mL/kg/h was started 5 min before clamping, respectively. Renal IRI was induced by clamping the left renal pedicle for 30 min. The hemodynamics, arterial blood gases, and plasma concentrations of creatinine and lactate were measured at baseline and 1, 2, and 3 h after declamping. Lung wet-to-dry ratio was measured. The mRNA expression of tumor necrosis factor (TNF)-alpha, interleukin (IL) 1 beta, and IL-6 and histologic localization of TNF-alpha in the kidney and lung were measured.Results: Renal IRI induced metabolic acidosis, pulmonary edema, increases in plasma concentrations of creatinine and lactate, and augmentation of the cytokine mRNA expression and histologic localization of TNF-alpha in the kidney and Renal IRI induced lung. ANP prevented IRI-induced metabolic acidosis, pulmonary edema, increases in creatinine, lactate, and the cytokine mRNA expression, attenuated histologic localization of TNF-alpha in the kidney and lung, and increased oxygenation.Conclusions: ANP has renoprotective and anti-inflammatory effects on the kidney and lung in a rat model of renal IRI, suggesting that ANP attenuates kidney-lung crosstalk. (C) 2014 Elsevier Inc. All rights reserved.