Deterioration of enhanced biological phosphorus removal by the domination of microorganisms without polyphosphate accumulation
Deterioration of enhanced biological phosphorus removal by the domination of microorganisms without polyphosphate accumulation
复制标题
DOI：
10.2166/wst.1994.0270
复制
发表时间：
1994-09
影响因子：
2.7
通讯作者：
H. Satoh;T. Mino;T. Matsuo
中科院分区：
文献类型：
--
作者：
H. Satoh;T. Mino;T. Matsuo
通过厌氧-好氧操作增强生物除磷并不总是成功的。在本研究中，研究了一个失败的强化生物除磷工艺的活性污泥中的微生物代谢，以阐明失败的原因。污泥中的优势微生物在厌氧条件下对乙酸盐或丙酸盐的吸收过程中消耗碳水化合物，积累聚羟基烷酸盐。但由于未利用聚磷酸盐，未观察到磷酸盐的显著释放。除了乙酰辅酶a外，消耗的碳水化合物还通过丙炔辅酶a转化为聚羟基烷酸盐，这表明微生物具有将糖酵解产生的磷酸烯醇丙酮酸或丙酮酸转化为丙炔辅酶a的酶。丙酸发酵被认为是糖酵解过程中过量产生的还原力的储存库；因此，在维持氧化还原平衡的同时，微生物能够从糖原而不是多磷酸盐中获得能量。聚磷积累菌与现有微生物代谢系统的差异可能为避免生物除磷效果的恶化提供线索。
Enhanced biological phosphorus removal by anaerobic-aerobic operation is not always achieved successfully. In this study, microbial metabolism in the activated sludge of a failed enhanced biological phosphorus removal process was investigated to clarify the cause of the failure. The dominant microorganisms in the sludge consumed carbohydrates in the uptake process of acetate or propionate under anaerobic conditions and accumulated polyhydroxyalkanaate. But significant release of phosphate was not observed because polyphosphate was not utilized. Consumed carbohydrates were found to have been converted to polyhydroxyalkanoate via propionyl-CoA in addition to acetyl-CoA, indicating that the microorganisms had enzymes to convert phosphoenolpyruvate or pyruvate produced in glycolysis to propionyl-CoA. The propionate fermentation was supposed to work as the sink of the reducing power excessively produced in glycolysis; thus while maintaining the redox balance, microorganisms were able to get energy not from polyphosphate but from glycogen. The difference in the metabolic systems between polyphosphate accumulating bacteria and the present microorganisms may give hints to avoid the deterioration of enhanced biological phosphorus removal.