Specificity of a nucleolar 2'-O-methyltransferase for RNA substrates.
Specificity of a nucleolar 2'-O-methyltransferase for RNA substrates.
复制标题
核仁 2-O-甲基转移酶对 RNA 底物的特异性。
DOI：
10.1016/s0006-291x(88)81119-7
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发表时间：
1988
影响因子：
3.1
通讯作者：
Eales,SJ
中科院分区：
文献类型：
--
作者：
Eichler,DC;Eales,SJ
在这项研究中，我们检测了从艾氏腹水癌细胞的核仁中分离出的核仁2‘-O-甲基转移酶的特异性。核仁甲基转移酶能够甲基化RNA的四个核苷中的每一个，然而，特定核苷上的甲基化水平因RNA的类型而异。动力学分析和多胺对甲基化的刺激都表明，RNA的结构对核仁2‘-O-甲基转移酶的区分性和明显的特异性起着关键作用。
In this study we examined the specificity of a nucleolar 2′-O-methyltransferase isolated from nucleoli of Ehrlich ascites tumor cells. The nucleolar methyltransferase was capable of methylating each of the four nucleosides of RNA, however, the level of methylation at a particular nucleoside varied with the type of RNA. Both kinetic analysis and the stimulation of methylation by polyamines suggested that the structure of RNA was critical in influencing the discrimination and apparent specificity of nucleolar 2′-O-methyltransferase.