Comparative proteomic profiling of patients with atopic dermatitis based on history of eczema herpeticum infection and Staphylococcus aureus colonization
Comparative proteomic profiling of patients with atopic dermatitis based on history of eczema herpeticum infection and Staphylococcus aureus colonization
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DOI：
10.1016/j.jaci.2010.10.033
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发表时间：
2011-01-01
影响因子：
14.2
通讯作者：
Leung, Donald Y. M.
中科院分区：
文献类型：
--
作者：
Broccardo, Carolyn J.;Mahaffey, Spencer;Leung, Donald Y. M.
背景：特应性皮炎（AD）是全世界普通人群中最常见的炎症性皮肤病，大多数患者都有金黄色葡萄球菌定植。疱疹性湿疹是一种播散性单纯疱疹病毒感染，发生在一小部分患者中。目的：目的是根据金黄色葡萄球菌定植状态和疱疹性湿疹病史对AD患者进行蛋白质组学分析。我们希望找到新的生物标志物，以改善疱疹性湿疹和金黄色葡萄球菌易感性的诊断和预测，并对疾病的发病机制提出新的假设。方法：对非特应性对照组的非病变皮肤和AD患者的病变和非病变皮肤进行皮肤贴敷。受试者根据疱疹性湿疹和金黄色葡萄球菌定植史进行分类。用质谱法分析蛋白质；诊断组比较蛋白表达差异有统计学意义。结果：与皮肤屏障相关的蛋白（聚丝蛋白-2、角膜粘连蛋白、粘连蛋白-1、粘连蛋白-1和转谷氨酰胺酶-3）和天然保湿因子（精氨酸酶-1、caspase-14和γ -谷氨酰环转移酶）的产生在有或没有疱疹性湿疹病史的AD患者的病变部位的表达水平显著低于非病变部位；耐甲氧西林金黄色葡萄球菌患者表皮脂肪酸结合蛋白表达水平显著升高。结论：这种无创、半定量的分析方法揭示了可能参与AD发病机制的新蛋白。参与生成天然保湿因子的皮肤屏障蛋白和酶的低表达可能进一步加剧屏障缺陷，使皮肤水分长期流失。表皮脂肪酸结合蛋白的高表达，特别是在耐甲氧西林金黄色葡萄球菌定植的患者中，可能通过类二十烷信号传导使炎症反应持续存在。[J] .中华过敏症杂志，2011;27(7):391 - 391。
Background: Atopic dermatitis (AD) is the most common inflammatory skin disorder in the general population worldwide, and the majority of patients are colonized with Staphylococcus aureus. Eczema herpeticum is a disseminated herpes simplex virus infection that occurs in a small subset of patients.Objectives: The goal was to conduct proteomic profiling of patients with AD based on S aureus colonization status and history of eczema herpeticum. We hoped to identify new biomarkers for improved diagnosis and prediction of eczema herpeticum and S aureus susceptibility and to generate new hypotheses regarding disease pathogenesis.Methods: Skin taping was performed on nonlesional skin of nonatopic control subjects and on lesional and nonlesional skin of patients with AD. Subjects were classified according to the history of eczema herpeticum and S aureus colonization. Proteins were analyzed by using mass spectrometry; diagnostic groups were compared for statistically significant differences in protein expression.Results: Proteins related to the skin barrier (filaggrin-2, corneodesmosin, desmoglein-1, desmocollin-1, and transglutaminase-3) and generation of natural moisturizing factor (arginase-1, caspase-14, and gamma-glutamyl cyclotransferase) were expressed at significantly lower levels in lesional versus nonlesional sites of patients with AD with and without history of eczema herpeticum; epidermal fatty acid-binding protein was expressed at significantly higher levels in patients with methicillin-resistant S aureus.Conclusion: This noninvasive, semiquantitative profiling method has revealed novel proteins likely involved in the pathogenesis of AD. The lower expression of skin barrier proteins and enzymes involved in the generation of the natural moisturizing factor could further exacerbate barrier defects and perpetuate water loss from the skin. The greater expression of epidermal fatty acid-binding protein, especially in patients colonized with methicillin-resistant S aureus, might perpetuate the inflammatory response through eicosanoid signaling. (J Allergy Clin Immunol 2011;127:186-93.)