Platforms for Bioorthogonal Oligonucleotide-templated Reactions: Translating Concepts into Devices.
Platforms for Bioorthogonal Oligonucleotide-templated Reactions: Translating Concepts into Devices.
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生物正交寡核苷酸模板反应平台：将概念转化为设备。
DOI：
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
S. Ladame
中科院分区：
文献类型：
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作者：
S. Pavagada;S. Ladame
DNA测序技术的指数级改进，以及相关成本的迅速下降，日益导致个性化基因组医学领域的扩张。特定脱氧核糖核酸（DNA）或核糖核酸（RNA）分子序列或拷贝数的变化是诊断、预后、分类和监测多种病理（尤其是癌症）的关键特征。能够检测这些变化的技术需要能够以高灵敏度和高特异性检测DNA或RNA的分析工具。基于生物正交寡核苷酸模板反应（OTRs）的传感已经被认为是满足这些标准的一种优雅的策略，并成功地用于体外和体内核酸的定量检测。在此，我们将重点关注最近的努力，将生物正交OTRs应用于临床有用的生物传感器，使用固定在定制材料和平台上或嵌入的探针。
The exponential improvements made in DNA sequencing technologies, together with the rapidly declining associated costs, has increasingly led to the expansion of the field of personalised genomic medicine. Changes in the sequence or copy number of specific deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) molecules represent key signatures for the diagnosis, prognosis, classification and monitoring of a broad range of pathologies, most notably cancer. Technologies that can detect these changes require analytical tools that can detect DNA or RNA with high sensitivity and high specificity. Sensing based on bioorthogonal oligonucleotide-templated reactions (OTRs) has been recognised as an elegant strategy that satisfies these criteria and was successfully used for the quantitative detection of nucleic acids both in vitro and in vivo. Herein, we will focus on recent efforts to implement bioorthogonal OTRs into clinically useful biosensors using probes immobilised on or embedded in customised materials and platforms.