The molecular clockwork of a protein-based circadian oscillator
The molecular clockwork of a protein-based circadian oscillator
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DOI：
10.1016/j.febslet.2009.11.021
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发表时间：
2009-12-17
期刊：
影响因子：
3.5
通讯作者：
O'Shea, Erin K.
中科院分区：
文献类型：
--
作者：
Markson, Joseph S.;O'Shea, Erin K.
蓝细菌Synechococcus elongatus PCC 7942的生物钟由蛋白质KaiA、KaiB和KaiC组成的核心振荡器控制。值得注意的是，KaiC磷酸化状态的昼夜节律振荡可以通过混合三种Kai蛋白和三磷酸腺苷在试管中重建。体外振荡器提供了一个定义明确的系统，其中实验可以与数学分析相结合，以了解高度稳健的生物振荡器的机制。在这篇评论中，我们总结了生物化学的启蛋白和检查模型，已提出解释振荡出现的振荡器的成分的属性。(c)2009年欧洲生物化学学会联合会。Elsevier B.V.出版，保留所有权利。
The circadian clock of the cyanobacterium Synechococcus elongatus PCC 7942 is governed by a core oscillator consisting of the proteins KaiA, KaiB, and KaiC. Remarkably, circadian oscillations in the phosphorylation state of KaiC can be reconstituted in a test tube by mixing the three Kai proteins and adenosine triphosphate. The in vitro oscillator provides a well-defined system in which experiments can be combined with mathematical analysis to understand the mechanism of a highly robust biological oscillator. In this Review, we summarize the biochemistry of the Kai proteins and examine models that have been proposed to explain how oscillations emerge from the properties of the oscillator's constituents.(c) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.