Effects of hyperbaric oxygen on GDNF expression and apoptosis in spinal cord injury
Effects of hyperbaric oxygen on GDNF expression and apoptosis in spinal cord injury
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DOI：
10.1097/00001756-200410250-00014
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发表时间：
2004-10
期刊：
影响因子：
1.7
通讯作者：
Yimin Yu;Y. Matsuyama;M. Yanase;S. Ito;Kayo Adachi;Kotaro Satake;N. Ishiguro;K. Kiuchi
中科院分区：
文献类型：
--
作者：
Yimin Yu;Y. Matsuyama;M. Yanase;S. Ito;Kayo Adachi;Kotaro Satake;N. Ishiguro;K. Kiuchi
探讨高压氧治疗对创伤性脊髓损伤后继发性损伤进展的影响。早期高压氧治疗可显著减少损伤后1天凋亡细胞的数量。然而，高压氧不影响巨噬细胞或活化的小胶质细胞的增殖。与对照组相比，高压氧组损伤1 d后神经胶质细胞系源性神经营养因子（GDNF）和诱导型一氧化氮合成酶（iNOS）的基因表达明显减弱。免疫组化染色证实下调。尽管GDNF基因下调，但早期高压氧治疗可能通过抑制iNOS基因有效抑制细胞凋亡进程。
The effects of hyperbaric oxygen treatment on the progress of secondary damage following traumatic spinal cord injury were investigated. The early onset of hyperbaric oxygen treatment significantly diminished the number of apoptotic cells 1 day after the injury. However, hyperbaric oxygen did not influence the proliferation of macrophages or activated microglia. The gene expression of glial cell line-derived neurotrophic factor (GDNF) and inducible nitric oxide synthetase (iNOS) was significantly attenuated 1 day after the injury in the hyperbaric oxygen groups compared with the control group. The down-regulation was confirmed by immunohistochemical staining. Early hyperbaric oxygen treatment was shown to effectively suppress the progress of apoptosis perhaps via the inhibition of iNOS gene despite the down-regulation of the GDNF gene.