Comparison of spectral variation from spectroscopy to spectral imaging
Comparison of spectral variation from spectroscopy to spectral imaging
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DOI：
10.1364/ao.46.001343
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发表时间：
2007-03-10
期刊：
影响因子：
1.9
通讯作者：
Mahadevan-Jansen, Anita
中科院分区：
文献类型：
--
作者：
Gebhart, Steven C.;Majumder, Shovan K.;Mahadevan-Jansen, Anita
光学活检已经显示出以高灵敏度和特异性区分正常和患病组织。基于光纤探针的光谱系统不提供必要的空间信息来有效地指导治疗，最终需要从基于探针的光谱到光谱成像的过渡。这种转变的荧光和漫反射线形状的影响进行了研究。光谱和成像之间的光谱线形状的固有差异的特点和许多这些差异可能是由于在两个系统之间的照明收集几何形状的转变。线形差异的灵敏度相对于样品吸收和散射的变化以及光纤探头设计的各种参数（例如，光纤直径、光束转向）。光谱线形状的差异被描述在组织内的光漫射和基于探针的成像照明收集几何形状的源-检测器分离距离的分布之间的相对关系。蒙特卡罗模拟被用来确定光纤配置，最大限度地减少两个系统之间的线形差异。总之，我们预测，光纤探头设计，…光谱成像几何结构和被设计为模拟基于探头的几何结构的光谱成像系统将难以实现，这指向对照明-收集几何结构的后验校正，以协调成像和基于探头的光谱线形状，或者对基于探头的和光谱成像系统的组织辨别准确度的独立评估。(c)2007年，美国光学学会。
Optical biopsy has been shown to discriminate between normal and diseased tissue with high sensitivity and specificity. Fiber-optic probe-based spectroscopy systems do not provide the necessary spatial information to guide therapy effectively, ultimately requiring a transition from probe-based spectroscopy to spectral imaging. The effect of such a transition on fluorescence and diffuse reflectance line shape is investigated. Inherent differences in spectral line shape between spectroscopy and imaging are characterized and many of these differences may be attributed to a shift in illumination-collection geometry between the two systems. Sensitivity of the line-shape disparity is characterized with respect to changes in sample absorption and scattering as well as to changes in various parameters of the fiber-optic probe design (e.g., fiber diameter, beam steering). Differences in spectral line shape are described in terms of the relative relationship between the light diffusion within the tissue and the distribution of source-detector separation distances for the probe-based and imaging illumination-collection geometries. Monte Carlo simulation is used to determine fiber configurations that minimize the line-shape disparity between the two systems. In conclusion, we predict that fiber-optic probe designs that.. c a spectral imaging geometry and spectral imaging systems designed to emulate a probe-based geometry will be difficult to implement, pointing toward a posteriori correction for illumination-collection geometry to reconcile imaging and probe-based spectral line shapes or independent evaluation of tissue discrimination accuracy for probe-based and spectral imaging systems. (c) 2007 Optical Society of America.