Generation of a novel Wolbachia infection in Aedes albopictus (Asian tiger mosquito) via embryonic microinjection.
Generation of a novel Wolbachia infection in Aedes albopictus (Asian tiger mosquito) via embryonic microinjection.
复制标题
通过胚胎显微注射在白纹伊蚊（亚洲虎蚊）中产生新型沃尔巴克氏体感染。
DOI：
10.1016/j.ibmb.2005.03.015
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发表时间：
2005
影响因子：
3.8
通讯作者：
Dobson,StephenL
中科院分区：
文献类型：
--
作者：
Xi,Zhiyong;Dean,JeffryL;Khoo,Cynthia;Dobson,StephenL
正在研究减少或阻断蚊子传播病原体的遗传策略，作为加强目前控制措施的一种手段。载体抑制和替换的策略是基于细胞内沃尔巴克氏体细菌，其天然存在于许多昆虫种群中。母系遗传的沃尔巴克氏体已经进化出多种机制来操纵宿主昆虫的繁殖和促进感染入侵。一种机制是细胞质不相容性（CI），通过这种机制，沃尔巴克氏体通过有效地使未感染的雌性绝育来促进感染传播。在先前的现场测试中，释放沃尔巴克氏体感染的雄性被用来抑制现场种群的尖音库蚊。另一种策略是利用沃尔巴克氏体作为载体，将所需的转基因驱动到载体种群中（种群替换）。基于沃尔巴克氏体的种群抑制和种群替代策略需要在蚊子中产生人工沃尔巴克氏体关联的能力。在这里，我们展示了一种技术转移沃尔巴克氏体（转染）在医学上重要的蚊子物种：白纹伊蚊（亚洲虎蚊）。用显微注射法将双感染的山羊胚细胞质转移到山羊胚上。白纹伊蚊品系转化为非共生品系。所得到的蚊子系是单一感染wAlbB沃尔巴克氏体类型的。人工产生的感染类型是未知的自然发生，并显示一个新的CI交叉类型和第一个已知的例子双向CI在伊蚊。我们讨论了应用蚊子控制策略和沃尔巴克氏体感染在AE的演变的结果。白纹伊蚊
Genetic strategies that reduce or block pathogen transmission by mosquitoes are being investigated as a means to augment current control measures. Strategies of vector suppression and replacement are based upon intracellular Wolbachia bacteria, which occur naturally in many insect populations. Maternally inherited Wolbachia have evolved diverse mechanisms to manipulate host insect reproduction and promote infection invasion. One mechanism is cytoplasmic incompatibility (CI) through which Wolbachia promotes infection spread by effectively sterilizing uninfected females. In a prior field test, releases of Wolbachia-infected males were used to suppress a field population of Culex pipiens. An additional strategy would employ Wolbachia as a vehicle to drive desired transgenes into vector populations (population replacement). Wolbachia-based population suppression and population replacement strategies require an ability to generate artificial Wolbachia associations in mosquitoes. Here, we demonstrate a technique for transferring Wolbachia (transfection) in a medically important mosquito species: Aedes albopictus (Asian tiger mosquito). Microinjection was used to transfer embryo cytoplasm from a double-infected Ae. albopictus line into an aposymbiotic line. The resulting mosquito line is single-infected with the wAlbB Wolbachia type. The artificially generated infection type is not known to occur naturally and displays a new CI crossing type and the first known example of bidirectional CI in Aedes mosquitoes. We discuss the results in relation to applied mosquito control strategies and the evolution of Wolbachia infections in Ae. albopictus.