Vasoregression Linked to Neuronal Damage in the Rat with Defect of Polycystin-2
Vasoregression Linked to Neuronal Damage in the Rat with Defect of Polycystin-2
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DOI：
10.1371/journal.pone.0007328
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发表时间：
2009-10-06
期刊：
影响因子：
3.7
通讯作者：
Hammes, Hans-Peter
中科院分区：
文献类型：
--
作者：
Feng, Yuxi;Wang, Yumei;Hammes, Hans-Peter
背景资料：神经元损伤与患病视网膜中的血管功能障碍相关，但由于缺乏合适的模型，其中与神经元损伤相关的血管消退在成熟的视网膜血管系统中启动，因此潜在的机制仍然存在争议。本研究旨在探讨突变型纤毛基因polycystin-2过表达的转基因大鼠（TGR）神经元损伤与血管通畅之间的时间关系。方法：观察TGR和对照大鼠血管退行性改变、神经胶质细胞变化和神经营养因子表达的时间进程。通过石蜡包埋垂直切片的定量形态测定法进行神经元变化的测定。血管细胞的组成和通畅性进行了评估，定量视网膜形态学的消化制剂。通过蛋白质印迹和免疫荧光评估胶质细胞活化。结果：1个月时，TGR大鼠外核细胞层（ONL）数量和厚度减少了31%（p
Background: Neuronal damage is correlated with vascular dysfunction in the diseased retina, but the underlying mechanisms remain controversial because of the lack of suitable models in which vasoregression related to neuronal damage initiates in the mature retinal vasculature. The aim of this study was to assess the temporal link between neuronal damage and vascular patency in a transgenic rat (TGR) with overexpression of a mutant cilia gene polycystin-2.Methods: Vasoregression, neuroglial changes and expression of neurotrophic factors were assessed in TGR and control rats in a time course. Determination of neuronal changes was performed by quantitative morphometry of paraffin-embedded vertical sections. Vascular cell composition and patency were assessed by quantitative retinal morphometry of digest preparations. Glial activation was assessed by western blot and immunofluorescence. Expression of neurotrophic factors was detected by quantitative PCR.Findings: At one month, number and thickness of the outer nuclear cell layers (ONL) in TGR rats were reduced by 31% (p