Differential proteomic analysis reveals the mechanism of Musa paradisiaca responding to salt stress
Differential proteomic analysis reveals the mechanism of Musa paradisiaca responding to salt stress
复制标题
差异蛋白质组分析揭示了香蕉响应盐胁迫的机制。
DOI：
10.1007/s11033-018-4564-2
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发表时间：
2019-02-01
影响因子：
2.8
通讯作者：
Zeng, Chuansheng
中科院分区：
文献类型：
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作者：
Ji, Fu-Sang;Tang, Lu;Zeng, Chuansheng
盐胁迫是影响香蕉产量和品质的重要非生物胁迫之一。为了解香蕉的耐盐机制，采用iTRAQ技术研究了巴西香蕉在60 mmol/L NaCl胁迫下不同持续时间的蛋白质组响应。与对照组（0 mmol/LNaCl处理）比较，共鉴定出77种DEPs，并将其分为9种功能类型。这四个主要类别涉及蛋白质合成和降解、光合作用、防御反应以及能量和碳水化合物代谢。结果表明，通过促进光合作用、蛋白质合成与降解、脂类代谢和次生代谢，使植物受到的伤害降低到可修复的水平。盐胁迫下ROS的积累对细胞有害，并导致抗氧化系统的上调。此外，为了科普盐胁迫损伤的细胞，PCD被用来去除受损的细胞。此外，细胞骨架可以在维持细胞和氧化还原稳态中发挥重要作用。不同种类功能蛋白丰度比的变化表明植物对盐胁迫有不同的响应机制。综上所述，所观察到的功能蛋白表达变化的目的是建立一个新的代谢过程的稳态平衡。据我所知，这是第一份调查M。通过蛋白质组学分析，研究了盐胁迫对天堂花的影响。
Salinity is one of the most important abiotic stresses, which affects the yield and quality of banana (Musa paradisiaca). To understand the salinity tolerance mechanisms of banana, the iTRAQ technique is employed to reveal the proteomic response of Brazil banana under different durations of 60 mmol/L NaCl stress. We have identified 77 DEPs and classified them into nine functional categories, compared with control (0 mmol/L NaCl treatment). The four major categories involve protein synthesis and degradation, photosynthesis, defense response, and energy and carbohydrate metabolism. The results indicate that photosynthesis, protein synthesis and degradation, lipid metabolism and secondary metabolism are promoted to limit damage to a repairable level. The accumulation of ROS under salt stress is harmful to cells and causes up-regulation of antioxidant systems. Furthermore, to cope with cells injured by salt stress, PCD is used to remove the damaged. Additionally, the cytoskeleton can play an important role in maintaining cellular and redox homeostasis. Different categories of functional proteins by changing the abundance ratio shows that plants have different mechanisms of response to salinity. Conclusively, Function of the observed changes in protein expression objective is to establish a new metabolic process of steady-state balance. To my knowledge, this is the first report that investigates responses of M. paradisiaca to salt stress by proteomic analysis.