17Beta-estradiol regulation of the mRNA expression of T-type calcium channel subunits: role of estrogen receptor alpha and estrogen receptor beta.
17Beta-estradiol regulation of the mRNA expression of T-type calcium channel subunits: role of estrogen receptor alpha and estrogen receptor beta.
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DOI：
10.1002/cne.21901
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发表时间：
2009-01-20
影响因子：
2.5
通讯作者：
Ronnekleiv, Oline K.
中科院分区：
文献类型：
--
作者：
Bosch, Martha A.;Hou, Jingwen;Fang, Yuan;Kelly, Niartin J.;Ronnekleiv, Oline K.
低电压激活的(T型)钙通道负责神经元的突发性放电和递质释放，并对脑垂体细胞的胞吐和激素分泌起重要作用。T型通道含有一个α1亚基，其中有3个亚型，即Cav3.1、3.2和3.3，每种亚型都有不同的动力学特征。尽管17β-雌二醇调节T型钙通道的表达和功能，但其分子机制尚不清楚。目前，我们利用实时定量聚合酶链式反应技术对C57BL/6小鼠下丘脑核团和垂体中提取的RNA进行定量检测，以阐明E2对Cav3.1、3.2和3.3亚基的调节作用。这三个亚基在下丘脑和垂体中均有表达。雌二醇组视前内侧区和弓状核Cav3.1和3.2mRNA表达增加，但CaV3.3mRNA表达无明显变化。在垂体中，E_2处理后Cav3.1升高，Cav3.2和3.3降低。为了验证经典雌激素受体(ER)是否参与了这一调节，我们以ERα和ERβ缺陷的C57BL/6小鼠为研究对象，探讨了雌二醇对T型通道亚型的影响。事实上，我们发现，E2诱导的下丘脑Cav3.1的增加依赖于ERα，而对Cav3.2的E2效应依赖于ERα和ERβ。然而，E_2在垂体的作用仅依赖于ERα的表达。E_2对T-型钙通道的强烈调节可能是E_2增加下丘脑神经元兴奋性和调节脑垂体分泌的重要机制。
Low voltage-activated (T-type) calcium channels are responsible for burst firing and transmitter release in neurons and are important for exocytosis and hormone secretion in pituitary cells. T-type channels contain an α1 subunit, of which there are three subtypes, Cav3.1, 3.2 and 3.3, and each subtype has distinct kinetic characteristics. Although 17β-estradiol modulates T-type calcium channel expression and function, little is known about the molecular mechanisms involved. Presently, we used real-time PCR quantification of RNA extracted from hypothalamic nuclei and pituitary in vehicle and E2-treated C57BL/6 mice to elucidate E2-mediated regulation of Cav3.1, 3.2 and 3.3 subunits. The three subunits were expressed in both the hypothalamus and the pituitary. E2 treatment increased the mRNA expression of Cav3.1 and 3.2, but not Cav3.3, in the medial preoptic area and the arcuate nucleus. In the pituitary, Cav3.1 was increased with E2-treatment and Cav3.2 and 3.3 were decreased. In order to examine whether the classical estrogen receptors (ERs) were involved in the regulation, we used ERα- and ERβ-deficient C57BL/6 mice and explored the effects of E2 on T-type channel subtypes. Indeed, we found that the E2-induced increase in Cav3.1 in the hypothalamus was dependent on ERα, whereas the E2 effect on Cav3.2 was dependent on both ERα and ERβ. However, the E2-induced effects in the pituitary were dependent on only the expression of ERα. The robust E2-regulation of the T-type calcium channels could be an important mechanism by which E2 increases the excitability of hypothalamic neurons and modulates pituitary secretion.
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影响因子：
4.8
作者：
Couse, JF;Lindzey, J;Korach, KS
通讯作者：
Korach, KS
影响因子：
3.5
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Asarian, L;Geary, N
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Geary, N
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16.2
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Kim, D;Song, I;Shin, HS
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Shin, HS
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ERICKSON, KR;RONNEKLEIV, OK;KELLY, MJ
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KELLY, MJ
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2.5
作者：
HUGUENARD, JR;MCCORMICK, DA
通讯作者：
MCCORMICK, DA