Synthesis and characterization of novel pH-, ionic strength and temperature- sensitive hydrogel for insulin delivery
Synthesis and characterization of novel pH-, ionic strength and temperature- sensitive hydrogel for insulin delivery
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DOI：
10.1016/j.polymer.2010.02.013
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发表时间：
2010-04-06
期刊：
影响因子：
4.6
通讯作者：
Akhter, Zareen
中科院分区：
文献类型：
--
作者：
Rasool, Nadia;Yasin, Tariq;Akhter, Zareen
以卡拉胶(KC)、丙烯酸(AA)为原料，用乙烯基三乙氧基硅烷(VTESi)制备了一系列新型硅烷交联水凝胶。过硫酸钾引发了反应物之间的接枝和共聚反应。此外，KC和VTESi的羟基缩合导致了交联。这项工作的新奇之处在于使用VTESi作为这种水凝胶组合物的交联剂。用傅里叶变换红外光谱对所制备的水凝胶进行了结构表征。光谱分析证实了制备的水凝胶中含有饲料组分。热重分析表明，无论是AA含量高还是交联剂含量高，水凝胶的稳定性都有所提高。本文报道了原料组成、pH值(缓冲液、非缓冲液)、电解介质和温度对水凝胶溶胀行为的影响。在低单体比(KC：AA=1：7)的水凝胶中，最有希望获得高溶胀比的水凝胶。该水凝胶在酸性和中性pH下的pH响应使其适合于药物输送应用。以蛋白质类药物胰岛素为模型药物。它需要在小肠内给药才能发挥适当的作用：因此，我们研究了它在模拟胃液和肠液中的体外释放行为。胰岛素在模拟胃液(SGF)中的释药规律可以忽略不计，在模拟肠液(SIF)中可以忽略不计的缓释。所得结果与该水凝胶的溶胀特性吻合较好。这种水凝胶的弱结构使其更适合于药物输送，因为它能够在中性pH下释放药物6h后崩解。(C)2010爱思唯尔有限公司。保留所有权利。
A series of novel silane crosslinked hydrogel was prepared from kappa carrageenan (KC), acrylic acid (AA) using vinyltriethoxysilane (VTESi). Potassium persulphate initiated the grafting and copolymerization reactions between reactants. In addition, the condensation of the hydroxyl groups of KC and VTESi resulted into crosslinking. Novelty of this work is the use of VTESi as crosslinker for such a composition of hydrogel. The structure of prepared hydrogels was characterized by Fourier transform infrared spectroscopy. The analysis of spectra confirmed the presence of feed components in the prepared hydrogels. Thermogravimetric analysis showed an increase in the stability of the hydrogels either having high AA contents or crosslinker amount. The effect of feed components, pH (buffer, non-buffer), electrolytic media and temperature on the swelling behaviour of the hydrogels is reported here.Most promising results with high swelling ratio were observed in hydrogel having low monomeric ratio (KC:AA = 1:7). pH response of this hydrogel in acidic and neutral pH makes it suitable for drug delivery application. Insulin, a protein based drug was selected as a model drug. It requires its delivery in small intestine for proper action: therefore its release behaviour was studied in-vitro in simulated stomach and intestinal fluids. The release profile of insulin showed negligible release in simulated gastric fluid (SGF) and sustained release in simulated intestinal fluid (SIF). The obtained results are in good agreement with the swelling response of this hydrogel. The weak structure of this hydrogel makes it preferable for drug delivery, as it is able to get crumbled after releasing the drug for 6 h at neutral pH. (C) 2010 Elsevier Ltd. All rights reserved.