Effects on ES-D3 Cell Differentiation of Three-dimensional Cell Culture System using with Collagen Gel Derived from Chum Salmon Sources
Effects on ES-D3 Cell Differentiation of Three-dimensional Cell Culture System using with Collagen Gel Derived from Chum Salmon Sources
复制标题
使用鲑鱼源胶原凝胶的三维细胞培养系统对 ES-D3 细胞分化的影响
DOI：
10.11223/jarde.5.81
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Masaaki Nakamura
中科院分区：
文献类型：
--
作者：
K. Imai;S. Kusakawa;A. Tanoue;Masaaki Nakamura
我们使用源自大马哈鱼的去端肽胶原凝胶制备了三维细胞培养系统。为了检查鲑鱼来源的胶原凝胶是否适合作为 ES-D3 细胞分化的支架，我们比较了在鲑鱼来源的胶原蛋白 (FC)、传统胶原蛋白凝胶 (CG) 和猪来源的胶原海绵 (CS) 中培养的细胞的分化程度。使用鲑鱼来源的胶原凝胶培养的细胞与通过添加 mLIF 抑制分化的对照细胞之间没有发现细胞分化存在显着差异。对于 FC，没有观察到 ES-D3 细胞的有效分化，而胶原凝胶和源自猪源的胶原海绵都支持细胞分化。使用ES细胞的研究结果已被证明对于ES细胞分化因子的研究是有用的。此外，这些研究结果可能为成体干细胞的细胞分化因子提供重要线索。为此，需要开发有利于 ES 细胞长期体外培养的支架。
We prepared a three-dimensional cell culture system using atelocollagen gel derived from chum salmon sources. To examine whether collagen gel derived from salmon is suitable as a scaffold for ES-D3 cell differentiation, we compared the degrees of differentiation of cells cultured in a salmon collagen (FC), a conventional collagen gel (CG), and a collagen sponge derived from porcine sources (CS).No significant differences in cell differentiation were noted between cells cultured with the collagen gel derived from salmon and the control cells, the differentiation of which was inhibited by adding mLIF. With FC, efficient differentiation of ES-D3 cells was not observed, whereas both the collagen gel supported cell differentiation and the collagen sponge derived from the porcine sources. The results of studies using ES cells have proven useful in studies of ES cell differentiation factors. Moreover, these study results may yield important clues as to cell differentiation factors for adult stem cells. To that end, the development of scaffolds that facilitate the in vitro culturing of ES cells for long periods is desirable.
胶原酶消化鲑鱼动脉胶原原纤维凝胶制备细胞片的方法
DOI：
--
发表时间：
2004
期刊：
Journal of Bioscience and Bioengineering 98・6
影响因子：
--
作者：
Nagai;N.;Yunoki;S.;Sakata.;M.;Tajima.;K.;Munekata;M.
通讯作者：
M.
影响因子：
2.8
作者：
Nagai, N;Yunoki, S;Munekata, M
通讯作者：
Munekata, M
鲑鱼去端肽胶原的支架。
DOI：
--
发表时间：
2006
期刊：
Regenerative Medicine 5-suppl.
影响因子：
--
作者：
Nagai;N.;Yunoki;S.;Mori;K.;Satoh;Y.;Tajima;K.;Munekata;M.
通讯作者：
M.