Effects of mechanical stretch on the functions of BK and L-type Ca2+ channels in vascular smooth muscle cells.
Effects of mechanical stretch on the functions of BK and L-type Ca2+ channels in vascular smooth muscle cells.
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DOI：
10.1016/j.jbiomech.2017.11.015
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发表时间：
2018-01
影响因子：
2.4
通讯作者：
Zhiguo Zhang;Yuqiao Wen;Jing Du;Yang Yu;Sisi Liu;Xiaoan Wu;Hucheng Zhao
中科院分区：
文献类型：
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作者：
Zhiguo Zhang;Yuqiao Wen;Jing Du;Yang Yu;Sisi Liu;Xiaoan Wu;Hucheng Zhao
众所周知，病理性增加的机械拉伸在高血压期间的血管重塑中起着关键作用。然而，拉伸如何调节血管平滑肌细胞离子通道的功能仍有待阐明。在这里，我们证明了机械拉伸对大电导钙、电压活化钾（BK）和l型Ca2+通道活性的影响。与5%拉伸（生理性）相比，15%拉伸（病理性）上调了VSMCs中l型Ca2+和BK通道的电流密度以及钙振荡的频率和幅度。与5%拉伸相比，15%拉伸也增加了BK通道的打开概率和平均打开时间。BK和l型Ca2+通道参与了机械拉伸调节的钙振荡。我们的研究结果表明，在高血压期间，病理性拉伸改变了BK和l型Ca2+通道的活性，并控制了VSMCs的钙振荡。
It is well recognized that pathologically increased mechanical stretch plays a critical role in vascular remodeling during hypertension. However, how the stretch modulates the functions of ion channels of vascular smooth muscle cells (VSMCs) remains to be elucidated. Here, we demonstrated the effects of mechanical stretch on the activity of large conductance calcium, voltage-activated potassium (BK) and L-type Ca2+channels. In comparison with 5% stretch (physiological), 15% stretch (pathological) upregulated the current density of L-type Ca2+and BK channels as well as the frequency and amplitude of calcium oscillation in VSMCs. 15% stretch also increased the open probability and mean open time of the BK channel compared with 5% stretch. BK and L-type Ca2+channels participated in the mechanical stretch-modulated calcium oscillation. Our results suggested that during hypertension, pathological stretch altered the activity of BK and L-type Ca2+channels and manipulated the calcium oscillation of VSMCs.