CYP1, a hypovirus-regulated cyclophilin, is required for virulence in the chestnut blight fungus.
CYP1, a hypovirus-regulated cyclophilin, is required for virulence in the chestnut blight fungus.
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DOI：
10.1111/j.1364-3703.2010.00665.x
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发表时间：
2011-04
影响因子：
4.9
通讯作者：
Chen B
中科院分区：
文献类型：
--
作者：
Chen MM;Jiang M;Shang J;Lan X;Yang F;Huang J;Nuss DL;Chen B
亲环素是在真核生物中高度保守的肽基脯氨酰顺反异构酶，是免疫抑制药物环孢菌素A（CsA）的细胞靶点。我们克隆了cyp1，亲环素A编码基因的植物病原真菌栗疫病菌寄生，并表明，该基因被下调的毒力衰减hypovirus感染后。通过构建cyp1缺失突变体进一步研究cyp1的功能。虽然野生型C.结果表明，Ep155菌株对CsA敏感，而Δcyp1菌株对CsA高度耐受，表明CYP1是CsA的作用靶点。cyp1的缺失导致接种到板栗茎上的毒力降低。转录分析显示，cyp1的缺失也降低了编码异源三聚体三磷酸鸟苷结合蛋白信号传导途径关键组分的基因的转录水平，这些关键组分对于感知环境信号至关重要，并参与了C.寄生虫发育和毒力。
Cyclophilins are peptidyl-prolyl cis–trans isomerases that are highly conserved throughout eukaryotes and are the cellular target of the immunosuppressive drug cyclosporin A (CsA). We cloned cyp1, a cyclophilin A-encoding gene in the phytopathogenic fungus Cryphonectria parasitica, and showed that this gene was downregulated following infection by a virulence-attenuating hypovirus. The function of cyp1 was further investigated by construction of a cyp1 deletion mutant. Although the wild-type C. parasitica strain EP155 was sensitive to CsA, the Δcyp1 strain was highly tolerant to CsA, indicating that CYP1 was the target of CsA. Deletion of cyp1 resulted in reduced virulence when inoculated to chestnut stems. Transcriptional analysis revealed that deletion of cyp1 also reduced transcript levels for genes encoding key components of the heterotrimeric guanosine triphosphate-binding protein signalling pathway that are essential for sensing environmental cues and are involved in C. parasitica development and virulence.
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