COMPLETE NUCLEOTIDE-SEQUENCES AND THE CHARACTERISTICS OF 2 HEPATITIS-B VIRUS MUTANTS CAUSING SEROLOGICALLY NEGATIVE ACUTE OR CHRONIC HEPATITIS-B
COMPLETE NUCLEOTIDE-SEQUENCES AND THE CHARACTERISTICS OF 2 HEPATITIS-B VIRUS MUTANTS CAUSING SEROLOGICALLY NEGATIVE ACUTE OR CHRONIC HEPATITIS-B
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DOI：
10.1002/jmv.1890450303
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发表时间：
1995-03-01
影响因子：
12.7
通讯作者：
SHIKATA, T
中科院分区：
文献类型：
--
作者：
UCHIDA, T;GOTOH, K;SHIKATA, T
用聚合酶链式反应从1例急性肝炎和1例慢性肝炎患者的血清中扩增出乙肝病毒(HBVDNA)。两名患者的血清乙肝表面抗原和乙肝核心抗体均为阴性，均曾被诊断为非甲非乙非丙非丁非戊型肝炎。核苷酸序列显示X基因编码区有8个核苷酸的缺失，产生C端截短的X蛋白，以及可能的增强子II/核心启动子元件的突变。此外，DR2在5‘末端还显示了T-to-C突变。X基因编码区内的这些突变必须抑制HBVDNA的复制和表达，这似乎是尽管存在乙肝病毒感染但缺乏血清学标志的原因；(C)1995年Wiley-Liss，Inc.
Hepatitis B virus (HBV) DNA was amplified by the polymerase chain reaction from the sera of a patient with acute hepatitis and a patient with chronic hepatitis. Both patients were negative for serum hepatitis B surface antigen and hepatitis B core antibodies and had been previously diagnosed as non-A, non-B, non-C, non-D, non-E hepatitis. The nucleotide sequence revealed an 8-nucleotide deletion in the X-gene coding region creating a C-terminally truncated X protein, and probable mutation of the enhancer II/core promoter element. In addition, DR2 showed a T-to-C mutation at the extreme 5'-terminus. These mutations within the X-gene coding region must suppress replication and expression of HBV DNA, and this seems to be responsible for absence of serological markers despite the presence of HBV infection; (C) 1995 Wiley-Liss, Inc.