Melittin stimulates Na entry, Na-K pump activity and DNA synthesis in quiescent cultures of mouse cells
Melittin stimulates Na entry, Na-K pump activity and DNA synthesis in quiescent cultures of mouse cells
复制标题
蜂毒肽刺激小鼠细胞静止培养物中的 Na 进入、Na-K 泵活性和 DNA 合成
DOI：
10.1016/0092-8674(81)90442-6
复制
发表时间：
1981
期刊：
影响因子：
64.5
通讯作者：
P. Pettican
中科院分区：
文献类型：
--
作者：
E. Rozengurt;T. Gelehrter;A. Legg;P. Pettican
蜂毒素是一种两亲性多肽，可使静止的瑞士3T3细胞的Na-K泵活性增加数倍。与其他生长因子一样，蜂毒素通过增加钠进入细胞内来增加泵的活性。相比之下，毒素不会引发其他早期反应。在促进离子通量的浓度下，蜂毒素刺激静止的小鼠细胞DNA合成，与胰岛素、表皮生长因子和SV40 BHK细胞释放的生长因子协同作用。相反，蜂毒素与佛波醇酯或加压素都不能协同作用。蜂毒素的细胞效应与离子流信号在静止细胞中启动有丝分裂的观点是一致的。
Melittin, an amphipatic polypeptide, increases several fold the activity of Na-K pump in quiescent Swiss 3T3 cells. As with other growth factors, melittin increases the activity of the pump by increasing Na entry into the cell. In contrast, other early responses are not elicited by the toxin. At concentrations that promote ion fluxes, melittin stimulates DNA synthesis in quiescent mouse cells acting synergistically with insulin, epidermal growth factor and with the growth factor released by SV40 BHK cells. In contrast, melittin does not interact synergistically with either phorbol esters or vasopressin. The cellular effects of melittin are consistent with the proposal that ion fluxes signal the initiation of mitogenesis in quiescent cells.