Modulation of Neutrophil Chemokine Receptors by Staphylococcus aureus Supernate
Modulation of Neutrophil Chemokine Receptors by Staphylococcus aureus Supernate
复制标题
金黄色葡萄球菌上清液对中性粒细胞趋化因子受体的调节
作者：
K. Veldkamp;H. J. Heezius;Jan Verhoef;J. A. Strijp;K. V. Kessel
摘要在以前的研究中，我们发现金黄色葡萄球菌上清液（SaS）是中性粒细胞和单核细胞的有效激动剂。为了进一步研究SaS的免疫调节作用，研究了其对不同中性粒细胞受体的作用。通过流式细胞术定量了SaS处理前后各种中性粒细胞受体的表达。我们发现，SaS治疗中性粒细胞导致C5 a和fMLP受体（均为蛇形受体）的特异性和总体下调，而其他受体完全不受影响。由于这两种受体都参与趋化性，我们测试了在钙流和趋化性测定中的SaS的效果。我们发现，与SaS预孵育废除了细胞内钙离子浓度的上升后，触发与fMLP和C5 a。我们还表明，SaS是一种有效的抑制中性粒细胞趋化fMLP和C5 a，但不干扰趋化白细胞介素-8。这些结果表明，S.金黄色葡萄球菌在细胞外产生毒力因子，其损害对感染部位的趋化性。
ABSTRACT In a previous study, we showed that Staphylococcus aureus supernate (SaS) is a potent agonist for both neutrophils and mononuclear cells. To further investigate the immunomodulating effects of SaS, the effect on different neutrophil receptors was studied. Expression of various neutrophil receptors, before and after treatment with SaS, was quantified by flow cytometry. We found that SaS treatment of neutrophils resulted in a specific and total downregulation of the C5a and the fMLP receptor, both serpentine receptors, while other receptors were totally unaffected. Since these two receptors are both involved in chemotaxis, we tested the effect of SaS in calcium flux and chemotaxis assays. We showed that preincubation with SaS abrogated the rise in intracellular calcium concentration upon triggering with fMLP and C5a. We also showed that SaS is a potent inhibitor of neutrophil chemotaxis towards fMLP and C5a, but does not interfere with chemotaxis towards interleukin-8. These findings indicate that S. aureus produces a virulence factor extracellularly, which impairs chemotaxis towards the infected site.
模拟选择素的原核肽对实验性脑膜炎的抗炎作用。
DOI：
10.1093/infdis/168.6.1422
发表时间：
1993
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Rozdzinski,E;Jones,T;Burnette,WN;Burroughs,M;Tuomanen,E
通讯作者：
Tuomanen,E
肽化学引诱剂受体的交叉脱敏。
DOI：
--
发表时间：
1994
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Tomhave,ED;Richardson,RM;Didsbury,JR;Menard,L;Snyderman,R;Ali,H
通讯作者：
Ali,H
B 族链球菌抑制补体第五种成分的趋化活性。
DOI：
--
发表时间：
1988
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Hill,HR;Bohnsack,JF;Morris,EZ;Augustine,NH;Parker,CJ;Cleary,PP;Wu,JT
通讯作者：
Wu,JT