Proteomic survey of metabolic pathways in rice
Proteomic survey of metabolic pathways in rice
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DOI：
10.1073/pnas.172183199
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发表时间：
2002-09-03
影响因子：
11.1
通讯作者：
Yates, JR
中科院分区：
文献类型：
--
作者：
Koller, A;Washburn, MP;Yates, JR
利用双向凝胶电泳法、串联质谱仪和多维蛋白质鉴定技术对水稻叶片、根和种子组织进行了系统的蛋白质组学分析，检测和鉴定了2528个独特的蛋白质，这是迄今为止最全面的蛋白质组探索。表达模式的比较显示表明，参与中央代谢途径的酶在所有组织中都存在，而代谢专门化反映在组织特异性酶补体的出现上。例如，检测到组织特异性和亚细胞室特异性的ADP葡萄糖焦磷酸化酶亚型，从而从蛋白质组学上证实了不同组织中不同淀粉池的生物合成和分解所涉及的不同调控机制的存在。此外，在种子样本中发现了几种先前确定的过敏性蛋白，这表明蛋白质组学方法有可能调查食物样本中过敏原的发生情况。
A systematic proteomic analysis of rice (Oryza sativa) leaf, root, and seed tissue using two independent technologies, two-dimensional gel electrophoresis followed by tandem mass spectrometry and multidimensional protein identification technology, allowed the detection and identification of 2,528 unique proteins, which represents the most comprehensive proteome exploration to date. A comparative display of the expression patterns indicated that enzymes involved in central metabolic pathways are present in all tissues, whereas metabolic specialization is reflected in the occurrence of a tissue-specific enzyme complement. For example, tissue-specific and subcellular compartment-specific isoforms of ADPglucose pyrophosphorylase were detected, thus providing proteomic confirmation of the presence of distinct regulatory mechanisms involved in the biosynthesis and breakdown of separate starch pools in different tissues. In addition, several previously characterized allergenic proteins were identified in the seed sample, indicating the potential of proteomic approaches to survey food samples with regard to the occurrence of allergens.