Characterization of the Drosophila lipid droplet subproteome
Characterization of the Drosophila lipid droplet subproteome
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DOI：
10.1074/mcp.m600011-mcp200
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发表时间：
2006-06-01
影响因子：
7
通讯作者：
Kuehnlein, Ronald P.
中科院分区：
文献类型：
--
作者：
Beller, Mathias;Riedel, Dietmar;Kuehnlein, Ronald P.
贮脂滴是细胞和生物体脂肪代谢包括能量稳态所必需的普遍细胞器。尽管他们显然简单的设计，他们提出参与越来越多的细胞过程，提出了一个问题，在何种程度上反映了复杂的细胞器蛋白质组组成的功能multifariousness。在这里，我们提出了248个蛋白质鉴定的亚蛋白质组分析使用的果蝇脂肪体组织的脂质储存液滴。除了先前已知的脂滴相关PAT（周磷脂，ADRP和TIP47）结构域蛋白和几种哺乳动物脂滴蛋白的同源物，本研究确定了一些蛋白质的不同的生物学功能，包括细胞内运输支持这些细胞器的动态和多方面的特点。我们进行了细胞内定位研究，选择新鉴定的亚蛋白质组成员在组织培养细胞和脂肪体细胞直接。结果表明，脂肪体细胞的脂滴是组合蛋白质组成。我们提出，单细胞内脂滴的子集的特征在于蛋白质的“邮政编码”，这反映了功能差异或特定的代谢状态。
Lipid storage droplets are universal organelles essential for the cellular and organismal lipometabolism including energy homeostasis. Despite their apparently simple design they are proposed to participate in a growing number of cellular processes, raising the question to what extent the functional multifariousness is reflected by a complex organellar proteome composition. Here we present 248 proteins identified in a subproteome analysis using lipid storage droplets of Drosophila melanogaster fat body tissue. In addition to previously known lipid droplet-associated PAT (Perilipin, ADRP, and TIP47) domain proteins and homologues of several mammalian lipid droplet proteins, this study identified a number of proteins of diverse biological function, including intracellular trafficking supportive of the dynamic and multifaceted character of these organelles. We performed intracellular localization studies on selected newly identified subproteome members both in tissue culture cells and in fat body cells directly. The results suggest that the lipid droplets of fat body cells are of combinatorial protein composition. We propose that subsets of lipid droplets within single cells are characterized by a protein '' zip code,'' which reflects functional differences or specific metabolic states.