In vivo visualization of the cochlear nerve and nuclei with fluorescent axonal tracers
In vivo visualization of the cochlear nerve and nuclei with fluorescent axonal tracers
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DOI：
10.1016/s0378-5955(01)00368-9
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发表时间：
2001-12-01
期刊：
影响因子：
2.8
通讯作者：
Laszig, R
中科院分区：
文献类型：
--
作者：
Marangos, N;Illing, RB;Laszig, R
近年来发展起来的多通道神经假体可以直接刺激中枢听觉通路。基本上，这些已经用于由2型神经纤维瘤病引起的全听力损失的病例，其中双侧听神经损伤阻止了更多的外周刺激。听觉脑干植入物(ABI)的电极载体被设计成放置在位于脑干外侧表面的耳蜗核复合体上。尽管肿瘤生长或手术前的解剖结构发生了改变，但正确的电极位置对于最大限度地利用ABI进行电刺激时产生的节距变化而不产生副作用至关重要。为了辅助近侧听神经和耳蜗核的识别，将无毒的荧光轴突示踪剂Fast Blue或Fluorogold注射到大鼠和爪哇猴的耳蜗内。应用示踪剂后4~7天，在适当的紫外光照射下，第八颅神经的标记、进入脑干的标记和听觉纤维进入耳蜗核的初级辐射在活脑上可显示为彩色荧光。进一步的组织学处理显示，在两个物种中都有逆行标记的神经元胞体。我们的结果表明，这种方法也可以用于人类，以帮助外科医生正确定位电极阵列。(C)2001 Elsevier Science B.V.保留所有权利。
In recent years multichannel neuroprostheses have been developed which directly stimulate the central auditory pathway. Substantially these have been used in cases of total hearing loss caused by neurofibromatosis type 2 where bilateral damage to the auditory nerve prevents more peripheral stimulation. The electrode carrier of the auditory brainstem implant (ABI) is designed to be placed on the cochlear nucleus complex residing at the lateral brainstem surface. Despite altered anatomy due to tumor growth or preceding surgery, correct electrode placement is essential to maximize the variety of pitch percept elicited during electrical stimulation with the ABI without producing side-effects. In order to assist intraoperative identification of the proximal auditory nerve and cochlear nuclei, the non-toxic fluorescent axonal tracers Fast Blue or Fluorogold were injected into the cochlea of rats and Java monkeys. Four to seven days after tracer application, labeling of the eighth cranial nerve, its entrance into the brainstem and the primary radiation of auditory fibers into the cochlear nucleus could be demonstrated as colored fluorescence on the living brain under appropriate ultraviolet illumination. Additional histological processing revealed groups of retrogradely labeled neuronal cell bodies in both species. Our results suggest that this method could also be used in humans in order to aid surgeons with the proper positioning of the electrode array. (C) 2001 Elsevier Science B.V. All rights reserved.