Microdiversity of the Vaginal Microbiome is Associated with Preterm Birth.
Microdiversity of the Vaginal Microbiome is Associated with Preterm Birth.
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阴道微生物组的微多样性与早产有关。
DOI：
10.1101/2023.01.13.523991
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Korem,Tal
中科院分区：
文献类型：
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作者：
Liao,Jingqiu;Shenhav,Liat;Urban,JuliaA;Serrano,Myrna;Zhu,Bin;Buck,GregoryA;Korem,Tal
早产（PTB）是新生儿发病和死亡的主要原因。阴道微生物群与PTB有关，但这种关联的机制尚不完全清楚。了解微生物对选择压力的遗传适应，特别是那些与宿主有关的压力，可能会对这些关联产生深刻的见解。在这里，我们分析了705份阴道样本的宏基因组数据，这些样本来自40名自然早产的女性和135名来自多组微生物组研究妊娠计划的足月对照组。我们发现，阴道微生物组妊娠结束早产表现出独特的遗传谱。它在物种水平上具有更大的遗传多样性，我们在另一个队列中验证了这一结果，并且具有更高的抗菌素抗性基因的丰富度和多样性，可能是由转导促进的。有趣的是，我们发现加德纳菌推动了这种更高的遗传多样性，特别是在怀孕的前半段。我们进一步提出证据表明，栀子花。脂质代谢相关基因的重组频率更高，纯化选择更强。总体而言，我们的群体遗传学分析揭示了阴道微生物组与PTB之间的关联，并表明作用于阴道微生物的进化过程可能在诸如PTB等不良妊娠结局中发挥作用。
Preterm birth (PTB) is the leading cause of neonatal morbidity and mortality. The vaginal microbiome has been associated with PTB, yet the mechanisms underlying this association are not fully understood. Understanding microbial genetic adaptations to selective pressures, especially those related to the host, may yield insights into these associations. Here, we analyze metagenomic data from 705 vaginal samples collected during pregnancy from 40 women who delivered preterm spontaneously and 135 term controls from the Multi-Omic Microbiome Study-Pregnancy Initiative. We find that the vaginal microbiome of pregnancies that ended preterm exhibited unique genetic profiles. It was more genetically diverse at the species level, a result which we validate in an additional cohort, and harbored a higher richness and diversity of antimicrobial resistance genes, likely promoted by transduction. Interestingly, we find thatGardnerellaspecies drove this higher genetic diversity, particularly during the first half of the pregnancy. We further present evidence thatGardnerellaspp. underwent more frequent recombination and stronger purifying selection in genes involved in lipid metabolism. Overall, our population genetics analyses reveal associations between the vaginal microbiome and PTB and suggest that evolutionary processes acting on vaginal microbes may play a role in adverse pregnancy outcomes such as PTB.