Role of SEPALLATA3 (SEP3) as a downstream gene of miR156-SPL3-FT circuitry in ambient temperature-responsive flowering
Role of SEPALLATA3 (SEP3) as a downstream gene of miR156-SPL3-FT circuitry in ambient temperature-responsive flowering
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DOI：
10.4161/psb.21366
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发表时间：
2012-01-01
影响因子：
2.9
通讯作者：
Ahn, Ji Hoon
中科院分区：
文献类型：
--
作者：
Lee, Jeong Hwan;Kim, Jae Joon;Ahn, Ji Hoon
SEPALLATA3 （SEP3）是决定开花时间和花器官身份的重要基因。虽然SEP3对花器官特性的调控已经了解很多，但其作为开花位点T（开花位点T）下游基因调控环境温度响应型开花的作用尚不清楚。本研究表明，作为SQUAMOSA启动子结合蛋白样3 （SPL3）和FT的下游基因，SEP3可以调节不同环境温度下的开花时间。SEP3过表达在23℃和16℃下表现为温度不敏感开花，这表明SEP3活性的改变影响了环境温度响应开花。然而，SEP3的损伤对环境温度响应开花没有明显影响。不同温度下叶片和茎尖区SPL3和FT活性的改变影响了SEP3的表达。这些结果表明，miR156-SPL3-FT通路直接或间接调控SEP3的表达，从而调控拟南芥的环境温度响应开花。
SEPALLATA3 (SEP3) is important in determining flowering time as well as floral organ identity. although much is known about the regulation of floral organ identity by SEP3, its role as a downstream gene of FLOWERING LOCUS T (FT) for the regulation of ambient temperature-responsive flowering is poorly understood. here, we show that SEP3 as a downstream gene of SQUAMOSA PROMOTER BINDING PROTEIN-LIKE3 (SPL3) and FT modulates the flowering time in response to different ambient temperatures. SEP3 overexpression showed temperature-insensitive flowering at 23 degrees C and 16 degrees C. this suggests that altered SEP3 activity affects ambient temperature-responsive flowering. however, a lesion in SEP3 did not obviously affect ambient temperature- responsive flowering. SEP3 expression was affected by altered SPL3 and FT activities in the leaf and shoot apical regions at different temperatures. these results suggest that the miR156-SPL3-FT circuitry directly or indirectly regulates SEP3 expression for the regulation of ambient temperature- responsive flowering in Arabidopsis.