Efficient nitrous oxide recovery from incineration leachate by a nosZ-deficient strain of Pseudomonas aeruginosa
Efficient nitrous oxide recovery from incineration leachate by a nosZ-deficient strain of Pseudomonas aeruginosa
复制标题
nosZ 缺陷铜绿假单胞菌菌株从焚烧渗滤液中高效回收一氧化二氮
DOI：
10.1016/j.biortech.2019.122371
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发表时间：
2020
影响因子：
11.4
通讯作者：
Holmes Dawn E.
中科院分区：
文献类型：
--
作者：
Nie Hanbing;Liu Xinying;Dang Yan;Ji Yanan;Sun Dezhi;Smith Jessica A.;Holmes Dawn E.
在这项研究中，从实验室规模的移动床生物膜反应器(MBBR)中回收氧化亚氮，处理部分硝化处理的渗滤液和添加AnosZ缺陷的铜绿假单胞菌菌株。批式培养实验表明，该菌株对游离亚硝酸的抑制阈值为0.016 mg/L，高于该阈值的游离亚硝酸浓度严重抑制了异化硝酸盐还原途径基因(NARG、NIR和NorB)的反硝化和转录。该MBBR的长期运行取得了较高的亚硝酸盐去除效率和N2O转化率(>；95%)。当MBBR进水经过部分硝化处理的渗滤液含有较高的亚硝酸盐浓度，并将排水比调整为30%时，产生的沼气中N2O占大部分(80%)。细菌群落分析表明，NosZ缺乏的假单胞菌仍具有代谢活性，并对反应器中的反硝化过程起主要作用。本研究为从垃圾渗滤液中回收N2O提供了一种很有前途的方法。
In this study, nitrous oxide was recovered from a lab-scale moving-bed biofilm reactor (MBBR) treating partial nitrification-treated leachate supplemented with anosZ-deficient strain of Pseudomonasaeruginosa. Batch culture tests with thenosZ-deficient strain determined that the threshold for free nitrous acid (FNA) inhibition was 0.016 mg/L and that FNA concentrations above this threshold severely inhibited denitrification and transcription of genes from the dissimilatory nitrate reduction pathway (narG,nirS, andnorB). High nitrite removal and N2O conversion efficiencies (>95%) were achieved with long-term operation of this MBBR. N2O accounted for the majority of biogas (80%) produced when the MBBR was fed partial nitrification-treated leachate with high nitrite concentrations and the drainage ratio was adjusted to 30%. Bacterial community analysis revealed that thenosZ-deficientPseudomonasstrain remained metabolically active and was primarily responsible for denitrification processes in the reactor. This study presents a promising method for N2O recovery from incineration leachate.