Effect of benzolamide on pH in the proximal tubules and peritubular capillaries of the rat kidney.
Effect of benzolamide on pH in the proximal tubules and peritubular capillaries of the rat kidney.
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苯甲酰胺对大鼠肾脏近端肾小管和肾小管周围毛细血管 pH 值的影响。
DOI：
10.1007/bf00580847
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Caflisch,CR
中科院分区：
文献类型：
--
作者：
Gennari,FJ;Johns,C;Caflisch,CR
用玻璃膜pH微电极研究苯乙酰胺对大鼠肾小管和肾小管周围毛细血管pH的影响。对照肾小管周围毛细血管的pH值为7.38±0.02(N=11)，显著低于对照动脉的pH值7.44±0.01，p<；0.05。近端肾小管早期的pH值为7.20±0.02(N=17)，远端肾小管的pH值为6.74±0.06(N=13)，碱性更强，p<；.001；两个部位的pH值都比肾小管周围毛细血管的酸性更强。苯甲酰胺治疗对动脉或肾小管周围毛细血管的pH无明显影响，但使早期近端肾小管的pH降低了0.54个pH单位，降至6.66±0.04(N=13)，p<；.001。晚期近端pH值也略有下降至6.57±0.03(N=14)，p<；0.02。这些结果支持了先前的观察结果，即苯甲酰胺处理显著降低了近端肾小管早期的pH，并进一步证明了近端肾小管中HCO3−的重吸收是通过分泌氢离子完成的。
The influence of carbonic anhydrase inhibition with benzolamide on proximal tubular and peritubular capillary pH was evaluated in the rat using a glass-membrane pH microelectrode. Control peritubular capillary pH, 7.38±.02 (N=11), was significantly lower than control arterial pH, 7.44±.01, p<.05. Early proximal tubular pH, 7.20±.02 (N=17), was more alkaline than late proximal tubular pH, 6.74±.06 (N=13), p<.001; pH at both sites was more acid than in the peritubular capillary, p<.001. Benzolamide treatment had no detectable effect on arterial or peritubular capillary pH, but reduced early proximal tubular pH by 0.54 pH units, to 6.66±.04 (N=13), p<.001. Late proximal pH also fell slightly to 6.57±.03 (N=14), p<.02. These results support previous observations indicating that benzoamide treatment decreases early proximal tubular pH dramatically, and provide further evidence that HCO3−reabsorption is accomplished by hydrogen ion secretion in the proximal tubule.