Oxonol dyes as monitors of membrane potential: The effect of viruses and toxins on the plasma membrane potential of animal cells in monolayer culture and in suspension
Oxonol dyes as monitors of membrane potential: The effect of viruses and toxins on the plasma membrane potential of animal cells in monolayer culture and in suspension
复制标题
Oxonol 染料作为膜电位监测器：病毒和毒素对单层培养和悬浮动物细胞质膜电位的影响
DOI：
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发表时间：
1985
影响因子：
5.6
通讯作者：
C. Pasternak
中科院分区：
文献类型：
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作者：
C. Bashford;G. Alder;M. Gray;K. Micklem;C. Christopher Taylor;P. Turek;C. Pasternak
阳离子、花青和阴离子氧杂菁类的光学指示剂用于评估悬浮液和单层培养物中动物细胞的质膜电位。通过使用 K+（在缬氨霉素存在下）或 H+（在羰基氰化物对三氟甲氧基苯腙；FCCP 存在下）的扩散电势来校准光学信号；对于不同的细胞类型，两类染料的质膜电位值相似，范围为 -40 至 -90 mV。添加溶血性仙台病毒或金黄色葡萄球菌 α-毒素可使细胞去极化并导致它们泄漏单价阳离子；这些作用被细胞外Ca2+拮抗。感染水泡性口炎或塞姆利基森林病毒的细胞在感染周期中会去极化；其他病毒感染对质膜电位没有影响。
Optical indicators of the cationic, cyanine and anionic oxonol classes were used to evaluate the plasma membrane potential of animal cells in suspension and in monolayer culture. The optical signals were calibrated by using diffusion potential either of K+ (in the presence of valinomycin) or of H+ (in the presence of carbonyl cyanide p‐trifluoromethoxyphenylhydrazone; FCCP); both classes of dye gave similar values of plasma membrane potential, in the range −40 to −90 mV for different cell types. Addition of haemolytic Sendai virus or Staphylococcus aureus α‐toxin depolarizes cells and causes them to leak monovalent cations; these effects are antagonized by extracellular Ca2+. Cells infected with vesicular stomatitis or Semliki Forest virus become depolarized during an infectious cycle; infection with other viruses was without affect on plasma membrane potential.